[HTML][HTML] Review of NAD (P) H-dependent oxidoreductases: Properties, engineering and application

LS Vidal, CL Kelly, PM Mordaka, JT Heap - Biochimica et Biophysica Acta …, 2018 - Elsevier
Abstract NAD (P) H-dependent oxidoreductases catalyze the reduction or oxidation of a
substrate coupled to the oxidation or reduction, respectively, of a nicotinamide adenine …

NADPH-generating systems in bacteria and archaea

SK Spaans, RA Weusthuis, J Van Der Oost… - Frontiers in …, 2015 - frontiersin.org
Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is an essential electron
donor in all organisms. It provides the reducing power that drives numerous anabolic …

Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

DC Volke, RA Martino, E Kozaeva, AM Smania… - Nature …, 2022 - nature.com
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Pseudomonas putida KT2440 strain metabolizes glucose through a cycle formed by enzymes of the Entner-Doudoroff, Embden-Meyerhof-Parnas, and pentose …

PI Nikel, M Chavarría, T Fuhrer, U Sauer… - Journal of Biological …, 2015 - ASBMB
The soil bacterium Pseudomonas putida KT2440 lacks a functional Embden-Meyerhof-
Parnas (EMP) pathway, and glycolysis is known to proceed almost exclusively through the …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

[HTML][HTML] Reserve flux capacity in the pentose phosphate pathway enables Escherichia coli's rapid response to oxidative stress

D Christodoulou, H Link, T Fuhrer, K Kochanowski… - Cell systems, 2018 - cell.com
To counteract oxidative stress and reactive oxygen species (ROS), bacteria evolved various
mechanisms, primarily reducing ROS through antioxidant systems that utilize cofactor …

Ultrahigh performance liquid chromatography− tandem mass spectrometry method for fast and robust quantification of anionic and aromatic metabolites

JM Buescher, S Moco, U Sauer, N Zamboni - Analytical chemistry, 2010 - ACS Publications
Quantification of metabolites is of pivotal relevance in biology, where it complements more
established omics techniques such as transcriptomics and proteomics. Here, we present a …

Rational design of a synthetic Entner–Doudoroff pathway for improved and controllable NADPH regeneration

CY Ng, I Farasat, CD Maranas, HM Salis - Metabolic engineering, 2015 - Elsevier
NADPH is an essential cofactor for the biosynthesis of several high-value chemicals,
including isoprenoids, fatty acid-based fuels, and biopolymers. Tunable control over all …

The Entner–Doudoroff pathway empowers Pseudomonas putida KT2440 with a high tolerance to oxidative stress

M Chavarría, PI Nikel, D Pérez‐Pantoja… - Environmental …, 2013 - Wiley Online Library
Glucose catabolism of P seudomonas putida is carried out exclusively through the E ntner–
D oudoroff (ED) pathway due to the absence of 6‐phosphofructokinase. In order to activate …

Ethanol production in syngas-fermenting Clostridium ljungdahlii is controlled by thermodynamics rather than by enzyme expression

H Richter, B Molitor, H Wei, W Chen… - Energy & …, 2016 - pubs.rsc.org
Acetogenic bacteria are attracting interest as biocatalysts in the biotechnology industry,
since they are able to ferment carbon monoxide (CO)-rich gases. Wild-type strains produce …