[HTML][HTML] Biosensor-based engineering of biosynthetic pathways
Highlights•Harnessing cellular processes to produce valuable metabolic products is of
growing economic importance.•However, biological complexity necessitates many attempts …
growing economic importance.•However, biological complexity necessitates many attempts …
[HTML][HTML] Advances and prospects of phenolic acids production, biorefinery and analysis
E Valanciene, I Jonuskiene, M Syrpas, E Augustiniene… - Biomolecules, 2020 - mdpi.com
Biotechnological production of phenolic acids is attracting increased interest due to their
superior antioxidant activity, as well as other antimicrobial, dietary, and health benefits. As …
superior antioxidant activity, as well as other antimicrobial, dietary, and health benefits. As …
Engineering an allosteric transcription factor to respond to new ligands
Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …
as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of …
[HTML][HTML] A genome-wide approach for identification and characterisation of metabolite-inducible systems
EKR Hanko, AC Paiva, M Jonczyk, M Abbott… - Nature …, 2020 - nature.com
Inducible gene expression systems are vital tools for the advancement of synthetic biology.
Their application as genetically encoded biosensors has the potential to contribute to …
Their application as genetically encoded biosensors has the potential to contribute to …
Enabling tools for high-throughput detection of metabolites: metabolic engineering and directed evolution applications
Abstract Within the Design-Build-Test Cycle for strain engineering, rapid product detection
and selection strategies remain challenging and limit overall throughput. Here we …
and selection strategies remain challenging and limit overall throughput. Here we …
Tailor-made transcriptional biosensors for optimizing microbial cell factories
B De Paepe, G Peters, P Coussement… - Journal of Industrial …, 2017 - academic.oup.com
Monitoring cellular behavior and eventually properly adapting cellular processes is key to
handle the enormous complexity of today's metabolic engineering questions. Hence …
handle the enormous complexity of today's metabolic engineering questions. Hence …
Advances in engineering and optimization of transcription factor-based biosensors for plug-and-play small molecule detection
Highlights•Transcription factor-based biosensors are powerful analytical synthetic biology
tools.•Biosensors can be used for detection, metabolic engineering, and protein …
tools.•Biosensors can be used for detection, metabolic engineering, and protein …
Computational Redesign of an ω-Transaminase from Pseudomonas jessenii for Asymmetric Synthesis of Enantiopure Bulky Amines
Q Meng, C Ramírez-Palacios, N Capra… - ACS …, 2021 - ACS Publications
ω-Transaminases (ω-TA) are attractive biocatalysts for the production of chiral amines from
prochiral ketones via asymmetric synthesis. However, the substrate scope of ω-TAs is …
prochiral ketones via asymmetric synthesis. However, the substrate scope of ω-TAs is …
Engineering Transcriptional Regulator Effector Specificity Using Computational Design and In Vitro Rapid Prototyping: Developing a Vanillin Sensor
ELC de los Santos, JT Meyerowitz, SL Mayo… - ACS synthetic …, 2016 - ACS Publications
The pursuit of circuits and metabolic pathways of increasing complexity and robustness in
synthetic biology will require engineering new regulatory tools. Feedback control based on …
synthetic biology will require engineering new regulatory tools. Feedback control based on …
Sensitive cells: enabling tools for static and dynamic control of microbial metabolic pathways
Highlights•Recently developed genome engineering tools facilitate pathway control.•Novel
genetic regulators enable multi-level static and dynamic control.•State-of-the-art applications …
genetic regulators enable multi-level static and dynamic control.•State-of-the-art applications …