An updated view on horseradish peroxidases: recombinant production and biotechnological applications

FW Krainer, A Glieder - Applied microbiology and biotechnology, 2015 - Springer
Horseradish peroxidase has been the subject of scientific research for centuries. It has been
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …

Engineering functional thermostable proteins using ancestral sequence reconstruction

RES Thomson, SE Carrera-Pacheco… - Journal of Biological …, 2022 - ASBMB
Natural proteins are often only slightly more stable in the native state than the denatured
state, and an increase in environmental temperature can easily shift the balance toward …

The thermostability and specificity of ancient proteins

LC Wheeler, SA Lim, S Marqusee, MJ Harms - Current opinion in structural …, 2016 - Elsevier
Highlights•Ancestral sequence reconstruction can reveal ancient protein properties.•Many
reconstructed Precambrian proteins exhibit elevated thermostability.•It is unclear whether …

[HTML][HTML] The impact of tandem duplication on gene evolution in Solanaceae species

YL Huang, LK Zhang, K Zhang, SM Chen, JB Hu… - Journal of Integrative …, 2022 - Elsevier
Whole genome duplication (WGD) and tandem duplication (TD) are important modes of
gene amplification and functional innovation, and they are common in plant genome …

Ancestral haloalkane dehalogenases show robustness and unique substrate specificity

P Babkova, E Sebestova, J Brezovsky… - …, 2017 - Wiley Online Library
Ancestral sequence reconstruction (ASR) represents a powerful approach for empirical
testing structure‐function relationships of diverse proteins. We employed ASR to predict …

Experimental recreation of the evolution of lignin-degrading enzymes from the Jurassic to date

I Ayuso-Fernández, AT Martínez… - Biotechnology for …, 2017 - Springer
Abstract Background Floudas et al.(Science 336: 1715) established that lignin-degrading
fungi appeared at the end of Carboniferous period associated with the production of the first …

A peroxidase purified from cowpea roots possesses high thermal stability and displays antifungal activity against Colletotrichum gloeosporioides and Fusarium …

FA Silva, LM Albuquerque, TF Martins… - Biocatalysis and …, 2022 - Elsevier
Plants encode specific class III peroxidases (POX) as large multigene families that are
involved in lignification, cell elongation, stress defense, and seed germination. However …

[图书][B] Advances in Medical Imaging, Detection, and Diagnosis

R Bawa, GF Audette, SR Bawa, B Patel, BD Johnson… - 2023 - books.google.com
Medical care is the most critical issue of our time and will be so for the foreseeable future. In
this regard, the pace and sophistication of advances in medicine in the past two decades …

An Integrated Strategy Based on 10-DAB Extraction and In Situ Whole-Cell Biotransformation of Renewable Taxus Needles to Produce Baccatin III

P Kou, Y Yu, H Wang, Y Zhang, Z Jin, F Yu - Molecules, 2024 - mdpi.com
Baccatin III is a crucial precursor in the biosynthesis pathway of paclitaxel. Its main sources
are extraction from Taxus or chemical synthesis using 10-deacetylbaccatin III (10-DAB) as …

An overview of immunoassays

C Murphy, S Gilgunn, R O'Kennedy - Immunoassays, 2017 - taylorfrancis.com
This chapter provides a detailed and practical introduction to immunoassays. It examines the
comprehensive guides to different immunoassay formats ranging from direct to competitive …