Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …

The advancements in biotechnology over time have led to an increase in the demand of
pure, soluble and functionally active proteins. Recombinant protein production has thus …

The most common approaches to improve soluble expression of heterologous proteins are
applications of molecular chaperones such as DnaK, DnaJ, GrpE, GroEL and GroES. The …

A single-chain variable fragment (scFv) antibody is a recombinant protein in which a peptide
linker connects the variable regions of the heavy chain and light chain. Due to its smaller …

Cyclin D1 is a key regulatory factor of the G1 to S transition during cell cycle progression.
Aberrant cyclin D gene amplification and abnormal protein expression have been linked to …

Human interferon gamma (hIFN-γ) plays a pivotal role as a soluble cytokine with diverse
functions in both innate and adaptive immunity. In a previous investigation, we pinpointed …

ABSTRACT Single Chain Variable Fragment (scFv), a small fragment of antibody can be
used to substitute the monoclonal antibody for diagnostic purposes. Production of scFv in …

Targeted therapy has been developed for increasing the efficacy of cancer treatment. The
first step in targeted therapy is to select the appropriate cell surface marker as targeting …

Background: Immune checkpoints are molecules that act as regulators of immune system
pathways. However, some tumor cells can express the ligands of immune checkpoints to …

Persistent infection with human papillomavirus type 16 (HPV16) causes the development of
cervical cancer. Escherichia coli is a cost-effective host successfully used to develop a …