Solid-state NMR dipolar and chemical shift anisotropy recoupling techniques for structural and dynamical studies in biological systems

L Liang, Y Ji, K Chen, P Gao, Z Zhao, G Hou - Chemical Reviews, 2022 - ACS Publications
With the development of NMR methodology and technology during the past decades, solid-
state NMR (ssNMR) has become a particularly important tool for investigating structure and …

Cu single atom nanozyme based high‐efficiency mild photothermal therapy through cellular metabolic regulation

M Chang, Z Hou, M Wang, D Wen, C Li… - Angewandte …, 2022 - Wiley Online Library
Upregulation of heat shock proteins (HSPs) drastically compromises the treatment effect of
mild photothermal therapy (PTT). Herein, we designed a polyporous Cu single atom …

ATP analogues for structural investigations: case studies of a DnaB helicase and an ABC transporter

D Lacabanne, T Wiegand, N Wili, MI Kozlova… - Molecules, 2020 - mdpi.com
Nucleoside triphosphates (NTPs) are used as chemical energy source in a variety of cell
systems. Structural snapshots along the NTP hydrolysis reaction coordinate are typically …

Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution

M Callon, AA Malär, S Pfister, V Římal… - Journal of Biomolecular …, 2021 - Springer
Progress in NMR in general and in biomolecular applications in particular is driven by
increasing magnetic-field strengths leading to improved resolution and sensitivity of the …

Protein NMR Spectroscopy at 150 kHz Magic‐Angle Spinning Continues To Improve Resolution and Mass Sensitivity

M Schledorn, AA Malär, A Torosyan, S Penzel… - …, 2020 - Wiley Online Library
Spectral resolution is the key to unleashing the structural and dynamic information contained
in NMR spectra. Fast magic‐angle spinning (MAS) has recently revolutionized the …

Protein structural dynamics by magic-angle spinning NMR

M Bonaccorsi, T Le Marchand, G Pintacuda - Current Opinion in Structural …, 2021 - Elsevier
Abstract Magic-Angle Spinning (MAS) Nuclear Magnetic Resonance (NMR) is a fast-
developing technique, capable of complementing solution NMR, X-ray crystallography, and …

Sedimentation yields long-term stable protein samples as shown by solid-state NMR

T Wiegand, D Lacabanne, A Torosyan… - Frontiers in molecular …, 2020 - frontiersin.org
Today, the sedimentation of proteins into a magic-angle spinning (MAS) rotor gives access
to fast and reliable sample preparation for solid-state Nuclear Magnetic Resonance (NMR) …

Exploring protein structures by DNP-enhanced methyl solid-state NMR spectroscopy

J Mao, V Aladin, X Jin, AJ Leeder… - Journal of the …, 2019 - ACS Publications
Although the rapid development of sensitivity-enhanced solid-state NMR (ssNMR)
spectroscopy based on dynamic nuclear polarization (DNP) has enabled a broad range of …

Use of paramagnetic systems to speed-up NMR data acquisition and for structural and dynamic studies

V Kocman, GM Di Mauro, G Veglia… - Solid state nuclear …, 2019 - Elsevier
NMR spectroscopy is a powerful experimental technique to study biological systems at the
atomic resolution. However, its intrinsic low sensitivity results in long acquisition times that in …

Flexible-to-rigid transition is central for substrate transport in the ABC transporter BmrA from Bacillus subtilis

D Lacabanne, C Orelle, L Lecoq, B Kunert… - Communications …, 2019 - nature.com
Abstract ATP-binding-cassette (ABC) transporters are molecular pumps that translocate
molecules across the cell membrane by switching between inward-facing and outward …