Hydroxyl radical protein footprinting (HRPF) coupled to mass spectrometry has been
successfully used to investigate a plethora of protein-related questions. The method, which …

Inevitable byproducts of aerobic metabolism are free radicals and other highly reactive
oxidants and nitrosants that are continuously formed within cells. Most cells generally …

Recent studies comparing the results of total organofluorine-combustion ion
chromatography (TOF-CIC) to targeted analysis of perfluoroalkyl and polyfluoroalkyl …

Knowledge of elaborate structures of protein complexes is fundamental for understanding
their functions and regulations. Although cross-linking coupled with mass spectrometry (MS) …

Collagen IV networks are ancient proteins of basement membranes that underlie epithelia in
metazoa from sponge to human. The networks provide structural integrity to tissues and …

MS/MS and associated database search algorithms are essential proteomic tools for
identifying peptides. Due to their widespread use, it is now time to perform a systematic …

For mass spectrometry-based proteomic analyses, electrospray ionization (ESI) and matrix-
assisted laser desorption/ionization (MALDI) are the commonly used ionization techniques …

Basement membranes are specialized extracellular matrices that underlie arterial wall
endothelial cells, with laminin being a key structural and biologically-active component …

Modern mass spectrometry-based proteomics can produce millions of peptide fragmentation
spectra, which are automatically identified in databases using sequence-specific b-or y-ions …

Protein footprinting based on hydroxyl radical-mediated modification and quantitative mass
spectroscopic analysis is a proven technique for examining protein structure, protein− ligand …