FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices

E Lerner, A Barth, J Hendrix, B Ambrose, V Birkedal… - Elife, 2021 - elifesciences.org
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

[HTML][HTML] Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

R Macháň, M Hof - Biochimica et Biophysica Acta (BBA)-Biomembranes, 2010 - Elsevier
Investigation of lipid lateral mobility in biological membranes and their artificial models
provides information on membrane dynamics and structure; methods based on optical …

[图书][B] Handbook of fluorescence spectroscopy and imaging: from ensemble to single molecules

M Sauer, J Hofkens, J Enderlein - 2010 - books.google.com
Providing much-needed information on fluorescence spectroscopy and microscopy, this
ready reference covers detection techniques, data registration, and the use of spectroscopic …

Pulsed interleaved excitation

BK Müller, E Zaychikov, C Bräuchle, DC Lamb - Biophysical journal, 2005 - cell.com
In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which
can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence …

Two‐focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements

T Dertinger, V Pacheco, I von der Hocht… - …, 2007 - Wiley Online Library
We present a new method to measure absolute diffusion coefficients at nanomolar
concentrations with high precision. Based on a modified fluorescence correlation …

Fluorescence Blinking and Photoactivation of All-Inorganic Perovskite Nanocrystals CsPbBr3 and CsPbBr2I

S Seth, N Mondal, S Patra… - The journal of physical …, 2016 - ACS Publications
Study of the emission behavior of all-inorganic perovskite nanocrystals CsPbBr3 and
CsPbBr2I as a function of the excitation power employing fluorescence correlation …

Practical guidelines for dual-color fluorescence cross-correlation spectroscopy

K Bacia, P Schwille - Nature protocols, 2007 - nature.com
Dual-color fluorescence cross-correlation spectroscopy (FCCS) allows for the determination
of molecular mobility and concentrations and for the quantitative analysis of molecular …

Molecular photobleaching kinetics of rhodamine 6G by one‐and two‐photon induced confocal fluorescence microscopy

C Eggeling, A Volkmer, CAM Seidel - ChemPhysChem, 2005 - Wiley Online Library
Under high‐excitation irradiance conditions in one‐and two‐photon induced fluorescence
microscopy, the photostability of fluorescent dyes is of crucial importance for the detection …

Performance of fluorescence correlation spectroscopy for measuring diffusion and concentration

J Enderlein, I Gregor, D Patra, T Dertinger… - …, 2005 - Wiley Online Library
Fluorescence correlation spectroscopy (FCS) has become an important tool for measuring
diffusion, concentration, and molecular interactions of cellular components. The …

Quantitative fluorescence imaging of protein diffusion and interaction in living cells

J Capoulade, M Wachsmuth, L Hufnagel… - Nature …, 2011 - nature.com
Diffusion processes and local dynamic equilibria inside cells lead to nonuniform spatial
distributions of molecules, which are essential for processes such as nuclear organization …