Behavior of enzymes under high pressure in food processing: mechanisms, applications, and developments

N Zheng, M Long, Z Zhang, S Du, X Huang… - Critical Reviews in …, 2024 - Taylor & Francis
High pressure processing (HPP) offers the benefits of safety, uniformity, energy-efficient, and
low waste, which is widely applied for microbial inactivation and shelf-life extension for …

Functional, structural properties and interaction mechanism of soy protein isolate nanoparticles modified by high-performance protein-glutaminase

N Zheng, M Long, Z Zhang, T Osire, Q Zan, H Zhou… - Food …, 2023 - Elsevier
Investigating the changes in the structural and functional properties of soy protein isolate
(SPI) modified by high-performance protein-glutaminase (PG) and understanding their …

Computer-Aided Reconstruction and Application of Bacillus halodurans S7 Xylanase with Heat and Alkali Resistance

H Zhou, Y Cai, M Long, N Zheng, Z Zhang… - Journal of Agricultural …, 2024 - ACS Publications
β-1, 4-Endoxylanase is the most critical hydrolase for xylan degradation during
lignocellulosic biomass utilization. However, its poor stability and activity in hot and alkaline …

Engineering of protein glutaminase for highly efficient modification of fish myofibrillar protein through structure-based and computational-aided strategy

W Leng, Y Li, X Liang, L Yuan, X Li, R Gao - Food Chemistry, 2024 - Elsevier
Abstract Protein glutaminase (PG; EC 3.5. 1.44) is a class of food-grade enzyme with the
potential to significantly improve protein functionality. However, its low catalytic activity and …

The effect of protein-glutaminase from Chryseobacterium proteolyticum on physicochemical and functional properties of high-temperature soybean meal protein

K Zhang, L Wang, Y Lyu, J Zhou, L Zhang… - International Journal of …, 2024 - Elsevier
Abstract Protein-glutaminase (EC 3.5. 1.44, PG) can significantly improve the functional
properties of food proteins. However, the low yield of PG has limited industrial applications …

Heterologous expression of protein-glutaminase in Escherichia coli Nissle 1917 for improved flavor properties of soy protein and its implications on high-moisture …

J Li, X Zhang, S Luo, X Wu, G Zhang, J Li, X Liu - Food Bioscience, 2024 - Elsevier
Beany flavor affects sensory experience of soy protein-based food. Deamidation is an
effective approach to improve the flavor of soy protein, but excessive hydrolysis has a …

Functional and Mechanistic Dissection of Protein Glutaminase PG3 and Its Rational Engineering for Enhanced Modification of Myofibrillar Proteins

W Leng, Y Li, L Yuan, X Li, R Gao - Journal of Agricultural and …, 2024 - ACS Publications
Protein glutaminases (PG; EC= 3.5. 1.44) are enzymes known for enhancing protein
functionality. In this study, we cloned and expressed the gene chryb 3 encoding protein …

Isothermal Compressibility Perturbation as a Protein Design Principle for T1 Lipase Stability–Activity Trade-Off Counteracting

N Zheng, L Gao, M Long, Z Zhang, C Zhu… - Journal of Agricultural …, 2023 - ACS Publications
Given the widely existing stability–activity trade-off in enzyme evolution, it is still a goal to
obtain enzymes embracing both high activity and stability. Herein, we employed an …

Directed Evolution of the UDP-Glycosyltransferase UGTBL1 for Highly Regioselective and Efficient Biosynthesis of Natural Phenolic Glycosides

C Zhang, Y Cai, Z Zhang, N Zheng… - Journal of Agricultural …, 2024 - ACS Publications
The O-glycosylation of polyphenols for the synthesis of glycosides has garnered substantial
attention in food research applications. However, the practical utility of UDP …

Discovery and mechanistic analysis of a novel source protein glutaminase PG5 and its potential application

W Leng, Y Li, X Liang, X Li, R Gao - Food Chemistry, 2024 - Elsevier
In this study, we successfully obtained a novel source protein glutaminase PG5 with specific
activity of 10.4 U/mg, good tolerance and broad substrate profile through big data retrieval …