Estrogens play a crucial role in spermatogenesis and estrogen receptor α knock-out male
mice are infertile. It has been demonstrated that estrogens significantly increase the speed …

Complementary molecules on the surface of both gametes are responsible for the
interaction of sperm protein receptors with zona pellucida (ZP) saccharide structures, and …

Defective mammalian spermatozoa are marked on their surface by proteolytic chaperone
ubiquitin. To identify potential ubiquitinated substrates in the defective spermatozoa, we …

17β-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and
egg development, sperm maturation—called capacitation—and sperm–egg communication …

Primary binding of the sperm to the zona pellucida (ZP) is one of the many steps necessary
for successful fertilization. Sperm bind ZP by means of membrane receptors which recognize …

Detection of enzymatic activity on gel electrophoresis, namely zymography, is a technique
that has received increasing attention in the last 10 years, according to the number of …

17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that
αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 …

The measurement of protein expression level plays a pivotal role in both biological and
medical studies. Housekeeping proteins, generally encoded by housekeeping genes are …

This study aimed to develop protocols for the extraction of sperm proteins from Moxotó goats
(Capra hircus) and to compare the resulting proteomic maps. The sperm proteins were …

Tato disertační práce byla zaměřena na multidisciplinární charakterizaci interakcí mezi
hormony a jejich receptory při kapacitaci spermií, která je finálním krokem maturace spermií …