RNA-based therapies, including RNA molecules as drugs and RNA-targeted small
molecules, offer unique opportunities to expand the range of therapeutic targets. Various …

Laboratory animal models play an important role in the study of human diseases. Using
appropriate animals is critical not only for basic research but also for the development of …

Stable gene expression in mammalian cells is a prerequisite for many in vitro and in vivo
experiments. However, either the integration of plasmids into mammalian genomes or the …

Transposons are mobile genetic elements evolved to execute highly efficient integration of
their genes into the genomes of their host cells. These natural DNA transfer vehicles have …

The widespread clinical implementation of gene therapy requires the ability to stably
integrate genetic information through gene transfer vectors in a safe, effective, and …

Emerging and well-known viral diseases remain one the most important global public health
threats. A better understanding of their pathogenesis and mechanisms of transmission …

Molecular medicine has entered a high-tech age that provides curative treatments of
complex genetic diseases through genetically engineered cellular medicinal products. Their …

Abnormal gene expression level or expression of genes containing deleterious mutations
are two of the main determinants which lead to genetic disease. To obtain a therapeutic …

Current transfection technologies lead to significant inter-clonal variations. Previously we
introduced a unique electrotransfection technology, Nanochannel-Electroporation (NEP) …

The pig has emerged as an important large animal model in biomedical and pharmaceutical
research. We describe a protocol for high-efficiency germline transgenesis and sustained …