Biosynthesis of human milk oligosaccharides: enzyme cascade and metabolic engineering approaches

J Meng, Y Zhu, H Wang, H Cao… - Journal of Agricultural and …, 2023 - ACS Publications
Human milk oligosaccharides (HMOs) have unique beneficial effects for infants and are
considered as the new gold standard for premium infant formula. They are a collection of …

Utilization of glycosyltransferases as a seamless tool for synthesis and modification of the oligosaccharides-A review

MY Ali, F Liaqat, MI Khazi, S Sethupathy… - International Journal of …, 2023 - Elsevier
Glycosyltransferases (GTs) catalyze the transfer of active monosaccharide donors to
carbohydrates to create a wide range of oligosaccharide structures. GTs display strong …

Human milk oligosaccharides as bioactive compounds in infant formula: recent advances and trends in synthetic methods

E Pérez-Escalante, S Alatorre-Santamaría… - Critical Reviews in …, 2022 - Taylor & Francis
Human milk oligosaccharides (HMO) have attracted great interest in recent years due to
their role in boosting infants and adults health. According to several in vitro, in vivo and …

Physiological effects, biosynthesis, and derivatization of key human milk tetrasaccharides, lacto-N-tetraose, and lacto-N-neotetraose

Y Zhu, G Luo, L Wan, J Meng, SY Lee… - Critical Reviews in …, 2022 - Taylor & Francis
Human milk oligosaccharides (HMOs) have recently attracted ever-increasing interest
because of their versatile physiological functions. In HMOs, two tetrasaccharides, lacto-N …

Recent progress in synthesis of carbohydrates with sugar nucleotide-dependent glycosyltransferases

L Na, R Li, X Chen - Current opinion in chemical biology, 2021 - Elsevier
Sugar nucleotide-dependent glycosyltransferases (GTs) are key enzymes that catalyze the
formation of glycosidic bonds in nature. They have been increasingly applied in the …

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2019–2020

DJ Harvey - Mass Spectrometry Reviews, 2023 - Wiley Online Library
This review is the tenth update of the original article published in 1999 on the application of
matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry to the analysis of …

A Redox‐Controlled Substrate Engineering Strategy for Site‐Specific Enzymatic Fucosylation

N Lu, Y Li, H Xia, K Zhong, C Jia, J Ye, X Liu… - Angewandte …, 2022 - Wiley Online Library
Fucosylation is one of the most common modifications of oligo‐N‐acetyllactosamine (oligo‐
LacNAc) glycans. However, none of known fucosyltransferases (FucTs) could install the α1 …

Exploring regioselective fucosylation catalyzed by bacterial glycosyltransferases through substrate promiscuity and acceptor-mediated glycosylation

HK Tseng, HK Wang, CY Wu, CK Ni, CC Lin - ACS Catalysis, 2023 - ACS Publications
Fucose in fucosylated glycans is primarily assembled at multiple sets of N-acetyllactosamine
(LN) and lacto-N-biose (LNB) units, which are known as Lewis antigens. The synthesis of …

Recent progress in fucosylated derivatives of lacto-N-tetraose and lacto-N-neotetraose

L Yang, Y Zhu, J Meng, W Zhang… - Critical Reviews in Food …, 2024 - Taylor & Francis
Human milk oligosaccharides (HMOs) have attracted considerable attention owing to their
unique physiological functions. Two important tetrasaccharides, lacto-N-tetraose (LNT) and …

Enzymatic modular synthesis of asymmetrically branched human milk oligosaccharides

Y Li, Y Li, Y Guo, C Chen, L Yang, Q Jiang, P Ling… - Carbohydrate …, 2024 - Elsevier
Human milk oligosaccharides (HMOs) are intricate glycans that promote healthy growth of
infants and have been incorporated into infant formula as food additives. Despite their …