Polymerase chain reaction in microfluidic devices

CD Ahrberg, A Manz, BG Chung - Lab on a Chip, 2016 - pubs.rsc.org
The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular
biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules …

Microfluidic DNA amplification—A review

Y Zhang, P Ozdemir - Analytica chimica acta, 2009 - Elsevier
The application of microfluidic devices for DNA amplification has recently been extensively
studied. Here, we review the important development of microfluidic polymerase chain …

Micro total analysis systems: latest achievements

J West, M Becker, S Tombrink, A Manz - Analytical chemistry, 2008 - ACS Publications
The field of micro total analysis systems (μTAS), or “lab on a chip”, has continued to gain
maturity. The importance of μTAS is reflected in both the growing number and the improved …

Multiplexed real-time polymerase chain reaction on a digital microfluidic platform

Z Hua, JL Rouse, AE Eckhardt, V Srinivasan… - Analytical …, 2010 - ACS Publications
This paper details the development of a digital microfluidic platform for multiplexed real-time
polymerase chain reactions (PCR). Liquid samples in discrete droplet format are …

Simplifying the complex: accessible microfluidic solutions for contemporary processes within in vitro diagnostics

NK Khosla, JM Lesinski, M Colombo, L Bezinge… - Lab on a Chip, 2022 - pubs.rsc.org
In vitro diagnostics (IVDs) form the cornerstone of modern medicine. They are routinely
employed throughout the entire treatment pathway, from initial diagnosis through to …

Single-molecule DNA amplification and analysis using microfluidics

C Zhang, D Xing - Chemical reviews, 2010 - ACS Publications
The broad availability of amplified DNA molecules enabled the development of many
powerful applications in molecular biology techniques. Currently, a variety of DNA …

Microfluidic-based nucleic acid amplification systems in microbiology

L Gorgannezhad, H Stratton, NT Nguyen - Micromachines, 2019 - mdpi.com
Rapid, sensitive, and selective bacterial detection is a hot topic, because the progress in this
research area has had a broad range of applications. Novel and innovative strategies for …

High-throughput real-time electrochemical monitoring of LAMP for pathogenic bacteria detection

M Safavieh, MU Ahmed, A Ng, M Zourob - Biosensors and Bioelectronics, 2014 - Elsevier
One of the significant challenges in healthcare is the development of point-of-care (POC)
diagnostics. POC diagnostics require low-cost devices that offer portability, simplicity in …

Microfluidic chip: next-generation platform for systems biology

X Feng, W Du, Q Luo, BF Liu - Analytica Chimica Acta, 2009 - Elsevier
Systems biology advocates the understanding of biology at the systems-level, which
requires massive information of correlations among individual components in complex …

The development of a portable buoyancy-driven PCR system and its evaluation by capillary electrophoresis

Z Li, Y Zhao, D Zhang, S Zhuang… - Sensors and Actuators B …, 2016 - Elsevier
Rapid, low cost, and portable polymerase chain reaction system (PCR) can advance the
diagnosis of infectious disease, especially for the third-world countries. Herein, we …