Beyond assembly: the increasing flexibility of single-molecule sequencing technology

PW Hook, W Timp - Nature Reviews Genetics, 2023 - nature.com
The maturation of high-throughput short-read sequencing technology over the past two
decades has shaped the way genomes are studied. Recently, single-molecule, long-read …

Agnostic sequencing for detection of viral pathogens

NPG Gauthier, SD Chorlton, M Krajden… - Clinical microbiology …, 2023 - Am Soc Microbiol
The advent of next-generation sequencing (NGS) technologies has expanded our ability to
detect and analyze microbial genomes and has yielded novel molecular approaches for …

Nanopore long-read-only metagenomics enables complete and high-quality genome reconstruction from mock and complex metagenomes

L Liu, Y Yang, Y Deng, T Zhang - Microbiome, 2022 - Springer
Background The accurate and comprehensive analyses of genome-resolved metagenomics
largely depend on the reconstruction of reference-quality (complete and high-quality) …

A signal processing and deep learning framework for methylation detection using Oxford Nanopore sequencing

MU Ahsan, A Gouru, J Chan, W Zhou… - Nature Communications, 2024 - nature.com
Oxford Nanopore sequencing can detect DNA methylations from ionic current signal of
single molecules, offering a unique advantage over conventional methods. Additionally …

The skin microbiome: current landscape and future opportunities

P Smythe, HN Wilkinson - International journal of molecular sciences, 2023 - mdpi.com
Our skin is the largest organ of the body, serving as an important barrier against the harsh
extrinsic environment. Alongside preventing desiccation, chemical damage and …

[HTML][HTML] Current concepts, advances, and challenges in deciphering the human microbiota with metatranscriptomics

T Ojala, AE Häkkinen, E Kankuri, M Kankainen - Trends in Genetics, 2023 - cell.com
Metatranscriptomics refers to the analysis of the collective microbial transcriptome of a
sample. Its increased utilization for the characterization of human-associated microbial …

Data Storage Using DNA

S Wang, X Mao, F Wang, X Zuo, C Fan - Advanced Materials, 2024 - Wiley Online Library
The exponential growth of global data has outpaced the storage capacities of current
technologies, necessitating innovative storage strategies. DNA, as a natural medium for …

Portable nanopore-sequencing technology: Trends in development and applications

P Chen, Z Sun, J Wang, X Liu, Y Bai, J Chen… - Frontiers in …, 2023 - frontiersin.org
Sequencing technology is the most commonly used technology in molecular biology
research and an essential pillar for the development and applications of molecular biology …

Efficient real-time selective genome sequencing on resource-constrained devices

PJ Shih, H Saadat, S Parameswaran… - …, 2023 - academic.oup.com
Background Third-generation nanopore sequencers offer selective sequencing or “Read
Until” that allows genomic reads to be analyzed in real time and abandoned halfway if not …

Combination of whole genome sequencing and metagenomics for microbiological diagnostics

S Purushothaman, M Meola, A Egli - International journal of molecular …, 2022 - mdpi.com
Whole genome sequencing (WGS) provides the highest resolution for genome-based
species identification and can provide insight into the antimicrobial resistance and virulence …