Modulating biomolecular condensates: a novel approach to drug discovery

DM Mitrea, M Mittasch, BF Gomes, IA Klein… - Nature Reviews Drug …, 2022 - nature.com
In the past decade, membraneless assemblies known as biomolecular condensates have
been reported to play key roles in many cellular functions by compartmentalizing specific …

Physical principles underlying the complex biology of intracellular phase transitions

JM Choi, AS Holehouse… - Annual review of …, 2020 - annualreviews.org
Many biomolecular condensates appear to form via spontaneous or driven processes that
have the hallmarks of intracellular phase transitions. This suggests that a common …

The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

S Lu, Q Ye, D Singh, Y Cao, JK Diedrich… - Nature …, 2021 - nature.com
The multifunctional nucleocapsid (N) protein in SARS-CoV-2 binds the~ 30 kb viral RNA
genome to aid its packaging into the 80–90 nm membrane-enveloped virion. The N protein …

Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu

M Poudyal, K Patel, L Gadhe, AS Sawner… - Nature …, 2023 - nature.com
Liquid-liquid phase separation (LLPS) has emerged as a crucial biological phenomenon
underlying the sequestration of macromolecules (such as proteins and nucleic acids) into …

Widespread occurrence of the droplet state of proteins in the human proteome

M Hardenberg, A Horvath, V Ambrus… - Proceedings of the …, 2020 - National Acad Sciences
A wide range of proteins have been reported to condensate into a dense liquid phase,
forming a reversible droplet state. Failure in the control of the droplet state can lead to the …

Prediction of liquid–liquid phase separating proteins using machine learning

X Chu, T Sun, Q Li, Y Xu, Z Zhang, L Lai, J Pei - BMC bioinformatics, 2022 - Springer
Background The liquid–liquid phase separation (LLPS) of biomolecules in cell underpins
the formation of membraneless organelles, which are the condensates of protein, nucleic …

Properties of stress granule and P-body proteomes

JY Youn, BJA Dyakov, J Zhang, JDR Knight… - Molecular cell, 2019 - cell.com
Stress granules and P-bodies are cytosolic biomolecular condensates that dynamically form
by the phase separation of RNAs and proteins. They participate in translational control and …

Defining the condensate landscape of fusion oncoproteins

S Tripathi, HK Shirnekhi, SD Gorman… - Nature …, 2023 - nature.com
Fusion oncoproteins (FOs) arise from chromosomal translocations in~ 17% of cancers and
are often oncogenic drivers. Although some FOs can promote oncogenesis by undergoing …

Liquid–liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau

S Ambadipudi, J Biernat, D Riedel… - Nature …, 2017 - nature.com
The protein Tau aggregates into tangles in the brain of patients with Alzheimer's disease. In
solution, however, Tau is intrinsically disordered, highly soluble, and binds to microtubules …

Screening membraneless organelle participants with machine-learning models that integrate multimodal features

Z Chen, C Hou, L Wang, C Yu, T Chen… - Proceedings of the …, 2022 - National Acad Sciences
Protein self-assembly is one of the formation mechanisms of biomolecular condensates.
However, most phase-separating systems (PS) demand multiple partners in biological …