RNA-binding proteins in human genetic disease

F Gebauer, T Schwarzl, J Valcárcel… - Nature Reviews …, 2021 - nature.com
RNA-binding proteins (RBPs) are critical effectors of gene expression, and as such their
malfunction underlies the origin of many diseases. RBPs can recognize hundreds of …

RNA-binding proteins in cardiovascular biology and disease: The beat goes on

M Völkers, T Preiss, MW Hentze - Nature Reviews Cardiology, 2024 - nature.com
Cardiac development and function are becoming increasingly well understood from different
angles, including signalling, transcriptional and epigenetic mechanisms. By contrast, the …

Nucleotide-amino acid π-stacking interactions initiate photo cross-linking in RNA-protein complexes

A Knörlein, CP Sarnowski, T de Vries, M Stoltz… - Nature …, 2022 - nature.com
Photo-induced cross-linking is a mainstay technique to characterize RNA-protein
interactions. However, UV-induced cross-linking between RNA and proteins at “zero …

The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins

LC Chu, P Arede, W Li, EC Urdaneta, I Ivanova… - Nature …, 2022 - nature.com
RNA-binding proteins play key roles in controlling gene expression in many organisms, but
relatively few have been identified and characterised in detail in Gram-positive bacteria …

RNxQuest: An Extension to the xQuest Pipeline Enabling Analysis of Protein–RNA Cross-Linking/Mass Spectrometry Data

CP Sarnowski, M Götze, A Leitner - Journal of Proteome …, 2023 - ACS Publications
Cross-linking and mass spectrometry (XL-MS) workflows are increasingly popular
techniques for generating low-resolution structural information about interacting …

An atlas of posttranslational modifications on RNA binding proteins

WE England, J Wang, S Chen, P Baldi… - Nucleic acids …, 2022 - academic.oup.com
RNA structure and function are intimately tied to RNA binding protein recognition and
regulation. Posttranslational modifications are chemical modifications which can control …

Photoactivatable ribonucleosides mark base-specific RNA-binding sites

JW Bae, S Kim, VN Kim, JS Kim - Nature Communications, 2021 - nature.com
RNA-protein interaction can be captured by crosslinking and enrichment followed by tandem
mass spectrometry, but it remains challenging to pinpoint RNA-binding sites (RBSs) or …

Npl3 functions in mRNP assembly by recruitment of mRNP components to the transcription site and their transfer onto the mRNA

P Keil, A Wulf, N Kachariya, S Reuscher… - Nucleic acids …, 2023 - academic.oup.com
RNA-binding proteins (RBPs) control every RNA metabolic process by multiple protein–RNA
and protein–protein interactions. Their roles have largely been analyzed by crude mutations …

Chloroacetamide‐Modified Nucleotide and RNA for Bioconjugations and Cross‐Linking with RNA‐Binding Proteins

M Brunderová, M Krömer, M Vlková… - Angewandte Chemie …, 2023 - Wiley Online Library
Reactive RNA probes are useful for studying and identifying RNA‐binding proteins. To that
end, we designed and synthesized chloroacetamide‐linked 7‐deaza‐ATP which was a …

Cross-linking and mass spectrometry as a tool for studying the structural biology of ribonucleoproteins

CP Sarnowski, M Bikaki, A Leitner - Structure, 2022 - cell.com
Cross-linking and mass spectrometry (XL-MS) workflows represent an increasingly popular
technique for low-resolution structural studies of macromolecular complexes. Cross-linking …