The CRISPR system is a revolutionary genome editing tool that has the potential to
revolutionize the field of cancer research and therapy. The ability to precisely target and edit …

The discovery of CRISPR has allowed site-specific genomic modification to become a reality
and this technology is now being applied in a number of human clinical trials. While this …

Systematic evaluation of the impact of genetic variants is critical for the study and treatment
of human physiology and disease. While specific mutations can be introduced by genome …

CRISPR-Cas9 genome editing has promising therapeutic potential for genetic diseases and
cancers, but safety could be a concern. Here we use whole genomic analysis by 10x linked …

Targeted eradication of transformed or otherwise dysregulated cells using monoclonal
antibodies (mAb), antibody–drug conjugates (ADC), T cell engagers (TCE), or chimeric …

Effective repolarization of macrophages has emerged as a promising approach for
anticancer therapy. However, there are very few studies on the effect of reprogramming …

Discovery of off-target CRISPR–Cas activity in patient-derived cells and animal models is
crucial for genome editing applications, but currently exhibits low sensitivity. We …

While a number of methods exist to investigate CRISPR off-target (OT) editing, few have
been compared head-to-head in primary cells after clinically relevant editing processes …

Controlled release of promoter-proximal paused RNA polymerase II (RNA Pol II) is crucial
for gene regulation. However, studying RNA Pol II pausing is challenging, as pause-release …

Ex vivo gene editing in hematopoietic stem and progenitor cells (HSPCs) represents a
promising curative treatment strategy for monogenic blood disorders. Gene editing using the …