Fluorescence correlation spectroscopy: the technique and its applications

O Krichevsky, G Bonnet - Reports on Progress in Physics, 2002 - iopscience.iop.org
Fluorescence correlation spectroscopy (FCS) is an experimental technique using statistical
analysis of the fluctuations of fluorescence in a system in order to decipher dynamic …

Fluorescence correlation spectroscopy: past, present, future

EL Elson - Biophysical journal, 2011 - cell.com
In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …

Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application

NO Petersen, PL Höddelius, PW Wiseman, O Seger… - Biophysical journal, 1993 - cell.com
Measurement of receptor distributions on cell surfaces is one important aspect of
understanding the mechanism whereby receptors function. In recent years, scanning …

Fluorescence-intensity distribution analysis and its application in biomolecular detection technology

P Kask, K Palo, D Ullmann… - Proceedings of the …, 1999 - National Acad Sciences
A methodology, fluorescence-intensity distribution analysis, has been developed for
confocal microscopy studies in which the fluorescence intensity of a sample with a …

Single-molecule biophysics: at the interface of biology, physics and chemistry

AA Deniz, S Mukhopadhyay… - Journal of the Royal …, 2008 - royalsocietypublishing.org
Single-molecule methods have matured into powerful and popular tools to probe the
complex behaviour of biological molecules, due to their unique abilities to probe molecular …

Fluorescence correlation spectroscopy

NL Thompson - Topics in fluorescence spectroscopy: Techniques, 2002 - Springer
Fluorescence correlation spectroscopy (FCS) is a technique in which temporal fluctuations
in the fluorescence measured from a sample of fluorescent molecules are analyzed to obtain …

Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy

Y Chen, JD Müller, QQ Ruan, E Gratton - Biophysical journal, 2002 - cell.com
We characterize the molecular properties of autofluorescence and transiently expressed
EGFP in the nucleus and in the cytoplasm of HeLa cells by fluorescence correlation …

Distribution of molecular aggregation by analysis of fluctuation moments.

H Qian, EL Elson - Proceedings of the National Academy of …, 1990 - National Acad Sciences
The fluorescence from an open volume of a solution of fluorescent molecules fluctuates as
the molecules randomly diffuse into and out of the volume. The distribution of degrees of …

Kinetic investigations by fluorescence correlation spectroscopy: the analytical and diagnostic potential of diffusion studies

P Schwille, J Bieschke, F Oehlenschläger - Biophysical chemistry, 1997 - Elsevier
This review demonstrates the large analytical and diagnostic potential of fluorescence
correlation spectroscopy applied to freely diffusing biomolecules in solution. All applications …

Resolving heterogeneity on the single molecular level with the photon-counting histogram

JD Müller, Y Chen, E Gratton - Biophysical journal, 2000 - cell.com
The diffusion of fluorescent particles through a small, illuminated observation volume gives
rise to intensity fluctuations caused by particle number fluctuations in the open observation …