Active transport between the nucleus and cytoplasm involves primarily three classes of
macromolecules: substrates, adaptors, and receptors. Some transport substrates bind …

Publisher Summary This chapter reviews and provides detailed protocols for the application
of immunofluorescence and other fluorescence-microscopic procedures to yeast. These …

We have cloned and sequenced the complete complementary DNA of the oestrogen
receptor (ER) present in the breast cancer cell line MCF-7. The expression of the ER cDNA …

Nucleolin is a 92 kd nucleolar protein implicated in regulating polymerase I transcription and
binding of preribosomal RNA. Another abundant nucleolar protein of 38 kd (B23/No38) is …

We have analyzed intracellular distributions of mRNAs for the cytoskeletal proteins actin,
vimentin, and tubulin by in situ hybridization. Although polyadenylated RNA was …

The basic carboxy terminus of p53 plays an important role in directing the protein into the
nuclear compartment. The C terminus of the p53 molecule contains a cluster of several …

The nuclear envelope defines a compartment boundary which is penetrated by pores that
mediate a remarkable transport process. Precursor RNAs are retained in the nucleus, while …

Nuclear protein transport is integral to eukaryotic cell processes such as differentiation,
transformation, and the control of gene expression. Although the targeting role of nuclear …

We have developed a large-scale screen to identify genes expressed at different times
during the life cycle of Saccharomyces cerevisiae and to determine the subcellular locations …

Protein transport into the nucleus is governed by the interaction of soluble transport factors
with their import substrates and nuclear pore complexes. Here, we identify a major distinct …