Non-covalent capillary coatings for protein separations in capillary electrophoresis

CA Lucy, AM MacDonald, MD Gulcev - Journal of Chromatography A, 2008 - Elsevier
Adsorption of proteins, particularly basic proteins onto fused silica capillaries severely
degrades capillary electrophoretic performance. This review provides a synopsis of the …

Electrokinetically controlled microfluidic analysis systems

L Bousse, C Cohen, T Nikiforov, A Chow… - Annual Review of …, 2000 - annualreviews.org
▪ Abstract Electrokinetic forces are emerging as a powerful means to drive microfluidic
systems with flow channel cross-sectional dimensions in the tens of micrometers and flow …

Capillary electrophoresis

WG Kuhr, CA Monnig - Analytical Chemistry, 1992 - ACS Publications
This is the second fundamental review of capillary electrophoresis (CE). The focus of this
review will center on the work in electrophoresis which has contributed to the devel-opment …

Capillaries modified by polyelectrolyte multilayers for electrophoretic separations

TW Graul, JB Schlenoff - Analytical Chemistry, 1999 - ACS Publications
Fused silica capillaries coated with thin films of physically adsorbed charged polymers are
employed for capillary zone electrophoretic separations. The coating is a polyelectrolyte …

Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications—updated and completely revised edition

F Krebs, H Zagst, M Stein, R Ratih, R Minkner… - …, 2023 - Wiley Online Library
This review is in support of the development of selective, precise, fast, and validated
capillary electrophoresis (CE) methods. It follows up a similar article from 1998, Wätzig H …

Strategies for capillary electrophoresis: method development and validation for pharmaceutical and biological applications

H Wätzig, M Degenhardt, A Kunkel - Electrophoresis, 1998 - Wiley Online Library
This review is in support of the development of selective, reproducible and validated
capillary electrophoretis (CE) methods. Focusing on pharmaceutical and biological …

Use of nanoparticles in capillary and microchip electrochromatography

C Nilsson, S Birnbaum, S Nilsson - Journal of Chromatography A, 2007 - Elsevier
Applications of nanoparticles are of rising interest in separation science, due to their
favorable surface-to-volume ratio as well as their applicability in miniaturization. A stationary …

High-efficiency, two-dimensional separations of protein digests on microfluidic devices

JD Ramsey, SC Jacobson, CT Culbertson… - Analytical …, 2003 - ACS Publications
High-efficiency, two-dimensional separations of tryptic digests were achieved using glass
microfluidic devices. Following micellar electrokinetic chromatography (MEKC) separations …

A microchip-based enzyme assay for protein kinase A

CB Cohen, E Chin-Dixon, S Jeong, TT Nikiforov - Analytical biochemistry, 1999 - Elsevier
A microchip-based enzyme assay for protein kinase A is described. The microchips were
prepared by standard photolithographic techniques. The assay reagents were placed in …

Noncovalent polycationic coatings for capillaries in capillary electrophoresis of proteins

E Cordova, J Gao, GM Whitesides - Analytical chemistry, 1997 - ACS Publications
The adsorption of proteins with net positive charges (p I> pH) on the walls of fused-silica
capillaries is a common problem in the analysis of proteins by capillary electrophoresis. This …