The classical genetic approach for exploring biological pathways typically begins by
identifying mutations that cause a phenotype of interest. Overexpression or misexpression of …

The myoD gene converts many differentiated cell types into muscle. MyoD is a member of
the basic-helix-loop-helix family of proteins; this 68-amino acid domain in MyoD is …

We have designed a system for targeted gene expression that allows the selective activation
of any cloned gene in a wide variety of tissueand cell-specific patterns. The gene encoding …

Abstract Development of endocrine cells in the endoderm involves Atonal and
Achaete/Scute-related basic helix-loop-helix (bHLH) proteins. These proteins also serve as …

Heterokaryons provide a model system in which to examine how tissue-specific phenotypes
arise and are maintained. When muscle cells are fused with nonmuscle cells, muscle gene …

Basic helix-loop-helix (bHLH) proteins are instrumental in determining cell type during
development. A bHLH protein, termed NeuroD, for neurogenic differentiation, has now been …

Crucial in the regulation of many developmental pathways is a recently identified class of
DNA binding proteins, designated as helix-loop-helix (HLH) proteins. HLH proteins are …

▪ Abstract For 600 million years, the two best-understood metazoan species, the nematode
Caenorhabditis elegans and the fruit fly Drosophila melanogaster, have developed …

Heterodimers between tissue-specific basic-helix-loop helix proteins and the gene products
of E2A play major roles in determining tissue-specific ceil fate. To understand the broad role …

In this report we provide four lines of evidence indicating that El2/E47-like proteins interact in
vivo with the myogenic HLH proteins MyoDand myogenin. First, cotransfection of MyoD and …