Plant tissue and organ cultures in vitro usually face technological challenges. When
submerged cultivation of plant cells in a controlled environment is desired, the characteristic …

The temporary immersion systems (TISs) have been widely used in plant biotechnology.
TISs have different advantages from the point of micropropagation and production of …

Walnuts (Juglans sp.) are allogamous species. Seed-derived plants are not always superior
to the selected parent. Clonal propagation of selected stock plants is an essential …

The cultivation of Vanilla planifolia is of great economic importance because vanillin, a
chemical valued in the food and cosmetics industry, is extracted from its pods. The …

Plant biotechnology can be used to conserve the germplasm of natural forests, and to
increase the productivity and sustainability of plantations. Both goals imply working with …

Culture in temporary immersion systems (TIS) is a valuable tool for the semi-automation of
high frequency somatic embryogenesis of coffee. This system allows the intermittent …

Key message This article summarizes the latest findings on the parameters affecting shoot
efficacy in TIS and the effects of intermittent immersion on the morpho-physiological …

A protocol has been developed for the propagation of Alnus glutinosa axillary shoots in
liquid medium. The explants were cultured in Woody Plant Medium supplemented with 0.1 …

Although the effectiveness of topolins in plant tissue culture systems has recently been
highlighted, there is a dearth of information on their interactions with auxins in relation to …

Although several studies have been reported on the micropropagation of the pistachio and
its rootstocks, to date none of them had been efficient on the mass production of these plants …