Recent advances in droplet microfluidics

Y Ding, PD Howes, AJ deMello - Analytical chemistry, 2019 - ACS Publications
Prominent within this technology set are droplet-based microfluidic systems, 3 in which
passive microfluidic structures are used to rapidly generate and manipulate subnanoliter …

Droplet-based digital PCR (ddPCR) and its applications

Y Hou, S Chen, Y Zheng, X Zheng, JM Lin - TrAC Trends in Analytical …, 2023 - Elsevier
As a new generation absolute quantitive PCR technique, droplet-based digital PCR
(ddPCR) provides the benefits of high sensitivity, precision, and reproducibility. A low-cost …

Pushbutton-activated microfluidic dropenser for droplet digital PCR

J Park, KG Lee, DH Han, JS Lee, SJ Lee… - Biosensors and …, 2021 - Elsevier
Here, we report a portable microfluidic device to generate and dispense droplets simply
operated by pushbutton for droplet digital polymerase chain reaction (ddPCR), which is …

A portable and cost-effective microfluidic system for massively parallel single-cell transcriptome profiling

C Liu, T Wu, F Fan, Y Liu, L Wu, M Junkin, Z Wang… - BioRxiv, 2019 - biorxiv.org
Single-cell technologies are becoming increasingly widespread and have been
revolutionizing our understanding of cell identity, state, diversity and function. However …

A portable droplet generation system for ultra-wide dynamic range digital PCR based on a vibrating sharp-tip capillary

Z He, J Wang, BJ Fike, X Li, C Li, BL Mendis… - Biosensors and …, 2021 - Elsevier
Monodisperse droplet has been widely used as a versatile tool in different disciplines
including biosensing. Existing methods still struggle to balance the droplet generation …

“SMART” digital nucleic acid amplification technologies for lung cancer monitoring from early to advanced stages

Y Ren, L Cao, M You, J Ji, Y Gong, H Ren, F Xu… - TrAC Trends in …, 2022 - Elsevier
Recently, liquid biopsy based on detection of circulating nucleic acid (NA) biomarkers from
body fluids has emerged as a promising strategy for lung cancer monitoring. Considering …

Microfluidic actuated and controlled systems and application for lab-on-chip in Space Life Science

Y Zhao, X Lv, X Li, N Rcheulishvili, Y Chen… - Space: Science & …, 2023 - spj.science.org
The use of space environment to carry out life science research is of great significance.
However, there are limitations in complex manipulation, research models based on …

Negative pressure provides simple and stable droplet generation in a flow-focusing microfluidic device

NA Filatov, AA Evstrapov, AS Bukatin - Micromachines, 2021 - mdpi.com
Droplet microfluidics is an extremely useful and powerful tool for industrial, environmental,
and biotechnological applications, due to advantages such as the small volume of reagents …

Picoliter Droplet Generation and Dense Bead-in-Droplet Encapsulation via Microfluidic Devices Fabricated via 3D Printed Molds

TD Anyaduba, JA Otoo, TS Schlappi - Micromachines, 2022 - mdpi.com
Picoliter-scale droplets have many applications in chemistry and biology, such as
biomolecule synthesis, drug discovery, nucleic acid quantification, and single cell analysis …

Droplet microfluidics enables tracing of target cells at the single-cell transcriptome resolution

Y Liu, S Wang, M Lyu, R Xie, W Guo, Y He, X Shi… - Bioengineering, 2022 - mdpi.com
The rapid promotion of single-cell omics in various fields has begun to help solve many
problems encountered in research, including precision medicine, prenatal diagnosis, and …