Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

The contrast and resolution of images obtained with optical microscopes can be improved
by deconvolution and computational fusion of multiple views of the same sample, but these …

Bioimaging data have significant potential for reuse, but unlocking this potential requires
systematic archiving of data and metadata in public databases. We propose draft metadata …

Confocal microscopy remains a major workhorse in biomedical optical microscopy owing to
its reliability and flexibility in imaging various samples, but suffers from substantial point …

Neuropil is a fundamental form of tissue organization within the brain, in which densely
packed neurons synaptically interconnect into precise circuit architecture,. However, the …

Light microscopes are the cell and developmental biologists'“best friend,” providing a means
to see structures and follow dynamics from the protein to the organism level. A huge …

During development, neurites and synapses segregate into specific neighborhoods or
layers within nerve bundles. The developmental programs guiding placement of neurites in …

Analyses across imaging modalities allow the integration of complementary spatiotemporal
information about brain development, structure, and function. However, systematic atlasing …

Light sheet fluorescence microscopy (LSFM) has become a method of choice for live
imaging because of its fast acquisition and reduced photobleaching and phototoxicity …

Systematic analysis of rich behavioral recordings is being used to uncover how circuits
encode complex behaviors. Here, we apply this approach to embryos. What are the first …