Cells alter their mechanical properties in response to their local microenvironment; this plays
a role in determining cell function and can even influence stem cell fate. Here, we identify a …

Stem cell-derived organoid technology is a powerful tool that revolutionizes the field of
biomedical research and extends the scope of our understanding of human biology and …

Fluorescence fluctuation spectroscopy (FFS) techniques provide information at the single-
molecule level with excellent time resolution. Usually applied at a single spot in a sample …

Single plane illumination microscopy based fluorescence correlation spectroscopy (SPIM-
FCS) is a new method for imaging FCS in 3D samples, providing diffusion coefficients …

Proteins within most macromolecular complexes or organelles continuously turn over. This
turnover results from association and dissociation reactions that are mediated by each of the …

Camera‐based fluorescence correlation spectroscopy (FCS) approaches allow the
measurement of thousands of contiguous points yielding excellent statistics and details of …

Concentration gradients of soluble proteins are believed to be responsible for control of
morphogenesis of subcellular systems, but the mechanisms that generate the spatial …

Functional fluorescence microscopy imaging (fFMI), a time-resolved (21 μs/frame) confocal
fluorescence microscopy imaging technique without scanning, is developed for quantitative …

Highlights•Imaging FCS is a quantitative bioimaging tool to quantify dynamics, interaction
and transport in live samples.•Imaging FCS provides multiplexing and spatio-temporal …

We describe a genetic AND gate for cell-targeted metabolic labeling and proteomic analysis
in complex cellular systems. The centerpiece of the AND gate is a bisected methionyl-tRNA …