Rational design of novel red‐shifted BRET pairs: Platforms for real‐time single‐chain protease biosensors
ST Gammon, VM Villalobos, M Roshal… - Biotechnology …, 2009 - Wiley Online Library
Bioluminescence resonance energy transfer (BRET) systems to date have been dominated
by use of blue-green Renilla luciferase (Rluc) as the energy donor. Although effective in …
by use of blue-green Renilla luciferase (Rluc) as the energy donor. Although effective in …
An autoinhibited coiled-coil design strategy for split-protein protease sensors
SS Shekhawat, JR Porter, A Sriprasad… - Journal of the American …, 2009 - ACS Publications
Proteases are widely studied as they are integral players in cell-cycle control and apoptosis.
We report a new approach for the design of a family of genetically encoded turn-on protease …
We report a new approach for the design of a family of genetically encoded turn-on protease …
[HTML][HTML] Comparison of static and microfluidic protease assays using modified bioluminescence resonance energy transfer chemistry
Background Fluorescence and bioluminescence resonance energy transfer (F/BRET) are
two forms of Förster resonance energy transfer, which can be used for optical transduction of …
two forms of Förster resonance energy transfer, which can be used for optical transduction of …
Bioanalytical systems based on bioluminescence resonance energy transfer using firefly luciferase
DV Smirnova, NN Ugarova - Combinatorial Chemistry & High …, 2015 - ingentaconnect.com
Bioanalytical systems based on the Bioluminescence Resonance Energy Transfer (BRET)
are widely used in fundamental biochemical studies, as well as for screening and analysis of …
are widely used in fundamental biochemical studies, as well as for screening and analysis of …
[HTML][HTML] Comparison of enhanced bioluminescence energy transfer donors for protease biosensors
H Dacres, M Michie, SC Trowell - Analytical Biochemistry, 2012 - Elsevier
Bioluminescence energy transfer (BRET) is a powerful tool for the study of protein–protein
interactions and conformational changes within proteins. We directly compared two recently …
interactions and conformational changes within proteins. We directly compared two recently …
Buffer enhanced bioluminescence resonance energy transfer sensor based on Gaussia luciferase for in vitro detection of protease
F Li, J Yu, Z Zhang, Z Cui, D Wang, H Wei… - Analytica chimica …, 2012 - Elsevier
Bioluminescence resonance energy transfer (BRET) has gained favors in recent years as a
detection technology for protease activity due to its extreme reliability, high sensitivity and …
detection technology for protease activity due to its extreme reliability, high sensitivity and …
Sequential bioluminescence resonance energy transfer–fluorescence resonance energy transfer-based ratiometric protease assays with fusion proteins of firefly …
BR Branchini, JC Rosenberg, DM Ablamsky… - Analytical …, 2011 - Elsevier
We report here the preparation of ratiometric luminescent probes that contain two well-
separated emission peaks produced by a sequential bioluminescence resonance energy …
separated emission peaks produced by a sequential bioluminescence resonance energy …
Bright bioluminescent BRET sensor proteins for measuring intracellular caspase activity
A den Hamer, P Dierickx, R Arts, JSPM de Vries… - ACS …, 2017 - ACS Publications
FRET-based caspase activity probes have become important tools to monitor apoptotic cell
signaling. However, their dependence on external illumination is incompatible with light …
signaling. However, their dependence on external illumination is incompatible with light …
[HTML][HTML] Multiplexing of multicolor bioluminescence resonance energy transfer
B Breton, E Sauvageau, J Zhou, H Bonin, C Le Gouill… - Biophysical journal, 2010 - cell.com
Bioluminescence resonance energy transfer (BRET) is increasingly being used to monitor
protein-protein interactions and cellular events in cells. However, the ability to monitor …
protein-protein interactions and cellular events in cells. However, the ability to monitor …
Chemically modified firefly luciferase is an efficient source of near-infrared light
BR Branchini, DM Ablamsky… - Bioconjugate …, 2010 - ACS Publications
Bioluminescence and bioluminescence resonance energy transfer (BRET) are two naturally
occurring light emission phenomena that have been adapted to a wide variety of important …
occurring light emission phenomena that have been adapted to a wide variety of important …