2, 3, 5-Triphenyltetrazolium chloride as a viability assay for immobilized plant cells

JL Iborra, J Guardiola, S Montaner, M Cánovas… - Biotechnology …, 1992 - Springer
Grape (V. vinifera L. cv Gamay Fréaux) cells were entrapped in calcium alginate beads. The
effect of bead diameter and gel concentration on the viability of the cells was checked. The …

Unsuitability of 2, 3, 5-triphenyl-2H-tetrazolium chloride (TTC) as a viability assay for plant cells in suspension

M Keßler, S Furusaki - Journal of chemical engineering of Japan, 1997 - jstage.jst.go.jp
The well-known viability assay with 2, 3, 5—triphenyl-ZH-tetrazolium chloride (TTC) is
applied to plant cell suspensions. In this paper it is shown that parameters (pH, TTC …

Immobilised plant cells: respiration and oxygen transfer

AC Hulst, J Tramper, P Brodelius… - Journal of Chemical …, 1985 - Wiley Online Library
The influence of support material (calcium alginate, x‐carrageenan and agarose), cell
loading and, in case of alginate, bead diameter on the rate of respiration of immobilised …

Measurement of cell viability in in vitro cultures

LA Castro-Concha, RM Escobedo… - Plant cell culture …, 2006 - Springer
An overview of the methods for assessing cell viability in in vitro cultures is presented. The
protocols of four of the most commonly used assays are described in detail, so the readers …

Growth kinetics of vitis vinifera cell suspension cultures: I. Shake flask cultures

MF Pépin, J Archambault, C Chavarie… - Biotechnology and …, 1995 - Wiley Online Library
Vitis vinifera cell suspension cultures carried out in shake flasks were closely examined for
biomass growth and cell division in relation to carbohydrate, NH4, NO3PO4, and dissolved …

Influence of substrate transport on the activity of immobilized Papaver somniferum cells

S Furusaki, T Nozawa, T Isohara, T Furuya - Applied microbiology and …, 1988 - Springer
Intraparticle diffusion resistance was studied for Papaver somniferum cells immobilized by
Ca alginate gel. In callus tissue, these plant cells convert codeinone to codeine. First, the …

Cultures of Nicotiana tabacum cells immobilized in calcium alginate gel beads coated with cell-free gel film

N Shibasaki-Kitakawa, Y Iizuka… - Journal of chemical …, 2001 - jstage.jst.go.jp
Nicotiana tabacum cells were immobilized in the Ca-alginate gel beads coated with the cell-
free gel film and then cultivated in the flask. The effects of the coating film thickness on …

Measurement of cell viability

LA Castro-Concha, RM Escobedo… - Plant Cell Culture …, 2012 - Springer
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Intraparticle cell growth and cell leakage in cultures of Nicotiana tabacum cells immobilized in calcium alginate gel beads

N Shibasaki‐Kitakawa, Y Iizuka… - Journal of Chemical …, 2000 - Wiley Online Library
Immobilized Nicotiona tabacum cells in calcium alginate gel beads were prepared under
various conditions and then were cultivated. The effects of different conditions of …

Effect of trisodium citrate treatment on hybridoma cell viability

GM Lee, JJ Gray, BØ Palsson - Biotechnology techniques, 1991 - Springer
Trisodium citrate has been widely used to dissolve calcium alginate beads in order to
estimate cell concentration in the beads. To obtain an accurate measurement of viable cell …