Improved characterization of EV preparations based on protein to lipid ratio and lipid properties
X Osteikoetxea, A Balogh, K Szabó-Taylor, A Németh… - PloS one, 2015 - journals.plos.org
In recent years the study of extracellular vesicles has gathered much scientific and clinical
interest. As the field is expanding, it is becoming clear that better methods for …
interest. As the field is expanding, it is becoming clear that better methods for …
An improved 96 well plate format lipid quantification assay for standardisation of experiments with extracellular vesicles
The field of extracellular vesicles (EVs) is an exponentially growing segment of biomedical
sciences. However, the problems of normalisation and quantification of EV samples have …
sciences. However, the problems of normalisation and quantification of EV samples have …
Mass-spectrometry-based molecular characterization of extracellular vesicles: lipidomics and proteomics
This review discusses extracellular vesicles (EVs), which are submicron-scale, anuclear,
phospholipid bilayer membrane enclosed vesicles that contain lipids, metabolites, proteins …
phospholipid bilayer membrane enclosed vesicles that contain lipids, metabolites, proteins …
Lipid profile characterization and lipoprotein comparison of extracellular vesicles from human plasma and serum
Y Sun, K Saito, Y Saito - Metabolites, 2019 - mdpi.com
Extracellular vesicles (EVs) consist of lipid bilayers, occur in various biofluids, and are
invaluable in biomarker screening. Liquid chromatography coupled with high-resolution …
invaluable in biomarker screening. Liquid chromatography coupled with high-resolution …
High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources
RA Haraszti, MC Didiot, E Sapp, J Leszyk… - Journal of …, 2016 - Taylor & Francis
Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for
use in diagnostics, therapeutics and drug delivery. However, little is known about the …
use in diagnostics, therapeutics and drug delivery. However, little is known about the …
Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin
RE Veerman, L Teeuwen, P Czarnewski… - Journal of …, 2021 - Wiley Online Library
Extracellular vesicles (EVs) are increasingly tested as therapeutic vehicles and biomarkers,
but still EV subtypes are not fully characterised. To isolate EVs with few co‐isolated entities …
but still EV subtypes are not fully characterised. To isolate EVs with few co‐isolated entities …
Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion …
JA Martínez‐Greene… - Journal of …, 2021 - Wiley Online Library
The molecular characterization of extracellular vesicles (EVs) has revealed a great
heterogeneity in their composition at a cellular and tissue level. Current isolation methods …
heterogeneity in their composition at a cellular and tissue level. Current isolation methods …
Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization subcommittee
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing
appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV …
appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV …
Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles' characteristics compared to precipitating agents
A Gámez-Valero, M Monguió-Tortajada… - Scientific reports, 2016 - nature.com
Extracellular vesicles (EVs) have become an attractive field among the scientific community.
Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation …
Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation …
The lipid composition of extracellular vesicles: applications in diagnostics and therapeutic delivery
S Ghadami, K Dellinger - Frontiers in Molecular Biosciences, 2023 - frontiersin.org
Extracellular vesicles (EVs), including exosomes, with nanoscale sizes, biological origins,
various functions, and unique lipid and protein compositions have been introduced as …
various functions, and unique lipid and protein compositions have been introduced as …