Improved characterization of EV preparations based on protein to lipid ratio and lipid properties

X Osteikoetxea, A Balogh, K Szabó-Taylor, A Németh… - PloS one, 2015 - journals.plos.org
In recent years the study of extracellular vesicles has gathered much scientific and clinical
interest. As the field is expanding, it is becoming clear that better methods for …

An improved 96 well plate format lipid quantification assay for standardisation of experiments with extracellular vesicles

T Visnovitz, X Osteikoetxea, BW Sódar… - Journal of …, 2019 - Taylor & Francis
The field of extracellular vesicles (EVs) is an exponentially growing segment of biomedical
sciences. However, the problems of normalisation and quantification of EV samples have …

Mass-spectrometry-based molecular characterization of extracellular vesicles: lipidomics and proteomics

S Kreimer, AM Belov, I Ghiran, SK Murthy… - Journal of proteome …, 2015 - ACS Publications
This review discusses extracellular vesicles (EVs), which are submicron-scale, anuclear,
phospholipid bilayer membrane enclosed vesicles that contain lipids, metabolites, proteins …

Lipid profile characterization and lipoprotein comparison of extracellular vesicles from human plasma and serum

Y Sun, K Saito, Y Saito - Metabolites, 2019 - mdpi.com
Extracellular vesicles (EVs) consist of lipid bilayers, occur in various biofluids, and are
invaluable in biomarker screening. Liquid chromatography coupled with high-resolution …

High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources

RA Haraszti, MC Didiot, E Sapp, J Leszyk… - Journal of …, 2016 - Taylor & Francis
Extracellular vesicles (EVs), including exosomes and microvesicles (MVs), are explored for
use in diagnostics, therapeutics and drug delivery. However, little is known about the …

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

RE Veerman, L Teeuwen, P Czarnewski… - Journal of …, 2021 - Wiley Online Library
Extracellular vesicles (EVs) are increasingly tested as therapeutic vehicles and biomarkers,
but still EV subtypes are not fully characterised. To isolate EVs with few co‐isolated entities …

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion …

JA Martínez‐Greene… - Journal of …, 2021 - Wiley Online Library
The molecular characterization of extracellular vesicles (EVs) has revealed a great
heterogeneity in their composition at a cellular and tissue level. Current isolation methods …

Methods for separation and characterization of extracellular vesicles: results of a worldwide survey performed by the ISEV rigor and standardization subcommittee

F Royo, C Théry, JM Falcón-Pérez, R Nieuwland… - Cells, 2020 - mdpi.com
Research on extracellular vesicles (EVs) is growing exponentially due to an increasing
appreciation of EVs as disease biomarkers and therapeutics, an expanding number of EV …

Size-Exclusion Chromatography-based isolation minimally alters Extracellular Vesicles' characteristics compared to precipitating agents

A Gámez-Valero, M Monguió-Tortajada… - Scientific reports, 2016 - nature.com
Extracellular vesicles (EVs) have become an attractive field among the scientific community.
Yet, a major challenge is to define a consensus method for EVs isolation. Ultracentrifugation …

The lipid composition of extracellular vesicles: applications in diagnostics and therapeutic delivery

S Ghadami, K Dellinger - Frontiers in Molecular Biosciences, 2023 - frontiersin.org
Extracellular vesicles (EVs), including exosomes, with nanoscale sizes, biological origins,
various functions, and unique lipid and protein compositions have been introduced as …