Vitrification of fully grown and growing porcine oocytes using germinal vesicle transfer

S Nakagawa, N Maedomari, K Kikuchi… - … of Reproduction and …, 2011 - jstage.jst.go.jp
The survival rate of vitrified germinal vesicle (GV) stage porcine oocytes is very low, and it is
not known if the vitrification damages the nucleus, cytoplasm or both. We have evaluated the …

Improved survival of vitrified in vivo-derived porcine embryos

H Ushijima, H Yoshioka, R Esaki… - … of Reproduction and …, 2004 - jstage.jst.go.jp
An efficient cryopreservation protocol for porcine morulae was investigated with three types
of vitrification having different cooling rates (Exp. 1). Survival of embryos vitrified after …

Vitrification of germinal vesicle and metaphase II swine oocytes

C Milovanov, E Bera Luțescu, S Marc Zarcula, T Keller… - 2016 - repository.uaiasi.ro
In vitro maturation of oocytes is a technique that implies extended cultivation of immature
oocytes, recovered by aspiring the cumulus-oocytes complexes from follicles, their …

Production of good-quality porcine blastocysts by in vitro fertilization of follicular oocytes vitrified at the germinal vesicle stage

T Somfai, J Noguchi, H Kaneko, M Nakai, M Ozawa… - Theriogenology, 2010 - Elsevier
We investigated survival, meiotic competence, cytoplasmic maturation, in vitro fertilization,
and development of immature porcine (Sus scrofa) oocytes cryopreserved by a modified …

Cryopreservation of immature bovine oocytes to reconstruct artificial gametes by germinal vesicle transplantation

AM Luciano, F Franciosi, V Lodde… - … in domestic animals, 2009 - Wiley Online Library
Contents Joining immature gamete cryopreservation and germinal vesicle transplantation
(GVT) technique could greatly improve assisted reproductive technologies in animal …

Porcine embryo production following in vitro fertilization and intracytoplasmic sperm injection from vitrified immature oocytes matured with a granulosa cell co-culture …

F Casillas, Y Ducolomb, AE Lemus, C Cuello… - Cryobiology, 2015 - Elsevier
This study was designed to evaluate the capacity of vitrified–warmed porcine immature
oocytes to mature and to be fertilized using in vitro fertilization or intracytoplasmic sperm …

82 COMPARISON OF THE SURVIVAL OF IN VITRO-DERIVED PORCINE OOCYTES AND EMBRYOS VITRIFIED BY OPEN PULLED STRAW, ELECTRON …

MH Ahn, HB Seok, ID Kim, DS Son - Reproduction, Fertility and …, 2003 - CSIRO Publishing
Various sample containers have been developed for ultra-rapid vitrification of oocytes and
embryos to minimize chilling and other injuries. In this study, the survival rates of in vitro …

Effects of cryopreservation on the developmental competence, ultrastructure and cytoskeletal structure of porcine oocytes

C Wu, R Rui, J Dai, C Zhang, S Ju, B Xie… - Molecular …, 2006 - Wiley Online Library
The purpose of this study was to determine ultrastructural and cytoskeletal changes that
result from vitrification of porcine germinal vesicle‐(GV‐) and meiosis II‐(MII‐) stage oocytes …

98 SUCCESSFUL PREGNANCIES FOLLOWING TRANSFER OF VITRIFIED PORCINE EMBRYOS DERIVED FROM IN VITRO-MATURED OOCYTES

K Hiruma, H Ueda, H Saito, C Tanaka… - Reproduction …, 2006 - CSIRO Publishing
To date only in vivo-produced embryos have successfully produced live piglets after
cryopreservation. In this study, we aimed to produce piglets from vitrified embryos derived …

82 IN VITRO SURVIVAL OF PORCINE BLASTOCYSTS VITRIFIED USING THE CRYOLOGIC VITRIFICATION METHOD

L Beebe, S McIlfatrick, R Ashman… - Reproduction, Fertility …, 2006 - CSIRO Publishing
Porcine embryo cryopreservation is an important technology for the storage and transport of
valuable genetic material. With many of the current vitrification and storage systems, such as …