Expression of theobgene and production of leptin have been examined on differentiation of
rat fibroblastic preadipocytes to adipocytes in primary culture. Preadipocytes were obtained …

mRNA levels of the ob gene product, leptin, were investigated by quantitative competitive RT‐
PCR in a mouse cell line (3T3‐L1) which can be induced to differentiate into adipocytes …

A 96-well plate format system is described for the in vitro culture and analysis of leptin
secretion by mature adipocytes. Cultured adipocytes secreted leptin in a linear fashion over …

A mutation within the obese gene was recently identified as the genetic basis for obesity in
the ob/ob mouse. The obese gene product, leptin, is a 16-kDa protein expressed …

Leptin has been shown to reduce body fat in vivo. Adipocytes express the leptin receptor;
therefore, it is realistic to expect a direct effect of leptin on adipocyte growth and metabolism …

Leptin, the product of theobgene, is expressed exclusively in adipose tissue. However,
adipocyte cell lines, such as 3T3-L1 adipocytes, have generally been reported to express …

The hormonal regulation of leptin mRNA expression and the association between leptin
expression and adipocyte differentiation were examined in primary cultures of porcine S‐V …

Obesity is caused by an increased mass of adipose tissue and enhances the risk for
diabetes mellitus and cardiovascular disorders. A part of the molecular mechanism of …

The aim of this study was to investigate the regulation of leptin expression and production in
cultured human adipocytes using the model of in vitro differentiated human adipocytes …

In the present study we have examined the effect of dexamethasone on ob gene mRNA
expression and leptin release from isolated human subcutaneous adipocytes …