A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase system

SC Andrews, BC Berks, J McClay, A Ambler… - …, 1997 - microbiologyresearch.org
The nucleotide sequence has been determined for a twelve-gene operon of Escherichia coli
designated the hyf operon (hyfABCDEFGHIR-focB). The hyf operon is located at 55.8-56.0 …

Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli

M Sauter, R Böhm, A Böck - Molecular microbiology, 1992 - Wiley Online Library
In‐frame deletions were introduced into each of the eight genes of the hyc operon coding for
products required for the formation of the formate hydrogenlyase (FHL) system. The …

Regulation of the Hydrogenase-4 Operon of Escherichia coli by the σ54-Dependent Transcriptional Activators FhlA and HyfR

DAG Skibinski, P Golby, YS Chang… - Journal of …, 2002 - Am Soc Microbiol
The hyf locus (hyfABCDEFGHIJ-hyfR-focB) of Escherichia coli encodes a putative 10-
subunit hydrogenase complex (hydrogenase-4 [Hyf]); a potential σ54-dependent …

Bacterial formate hydrogenlyase complex

JS McDowall, BJ Murphy, M Haumann… - Proceedings of the …, 2014 - National Acad Sciences
Under anaerobic conditions, Escherichia coli can carry out a mixed-acid fermentation that
ultimately produces molecular hydrogen. The enzyme directly responsible for hydrogen …

Expression and Regulation of a Silent Operon, hyf, Coding for Hydrogenase 4 Isoenzyme in Escherichia coli

WT Self, A Hasona, KT Shanmugam - Journal of bacteriology, 2004 - Am Soc Microbiol
On the basis of hyf-lacZ fusion studies, the hyf operon of Escherichia coli, noted for encoding
the fourth hydrogenase isoenzyme (HYD4), is not expressed at a significant level in a wild …

Identification and sequence analysis of the gene encoding the transcriptional activator of the formate hydrogenlyase system of Escherichia coli

V Schlensog, A Böck - Molecular microbiology, 1990 - Wiley Online Library
Through complementation of a trans‐acting regulatory mutation a gene has been cloned
whose product is required for the formate induction of the anaerobic expression of the …

Analysis of the hydA locus of Escherichia coli: two genes (hydN and hypF) involved in formate and hydrogen metabolism

T Maier, U Binder, A Böck - Archives of microbiology, 1996 - Springer
The hydA locus of Escherichia coli is known to encode some function necessary for
formation of hydrogenase activity. The locus contains two open reading frames, hydN and …

Differential expression of hydrogenase isoenzymes in Escherichia coli K-12: evidence for a third isoenzyme

RG Sawers, SP Ballantine, DH Boxer - Journal of bacteriology, 1985 - Am Soc Microbiol
The cellular contents of the nickel-containing, membrane-bound hydrogenase isoenzymes 1
and 2 (hydrogenases 1 and 2) were analyzed by crossed immunoelectrophoresis. Their …

[HTML][HTML] The roles of hydrogenases 3 and 4, and the F0F1-ATPase, in H2 production by Escherichia coli at alkaline and acidic pH

K Bagramyan, N Mnatsakanyan, A Poladian… - FEBS letters, 2002 - Elsevier
The hyc operon of Escherichia coli encodes the H2-evolving hydrogenase 3 (Hyd-3)
complex that, in conjunction with formate dehydrogenase H (Fdh-H), constitutes a …

Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli

S Lutz, A Jacobi, V Schlensog, R Böhm… - Molecular …, 1991 - Wiley Online Library
The 58/59 min region of the Escherichia coli chromosome contains two divergently oriented
gene clusters coding for proteins with a function in hydrogenase formation. One cluster (the …