Rodent models have been essential to understanding the immune‐mediated failure of aortic
valve allografts (AVAs). Decellularization has been proposed to reduce the immunogenicity …

OBJECTIVES: Decellularization of aortic valve allografts in advance of transplantation is a
promising approach to overcome immune-induced early graft failure. In this study the effects …

OBJECTIVES: Cryopreserved valve allografts used in congenital cardiac surgery are
associated with a significant cellular and humoral immune response. This might be reduced …

Background Various detergent‐based protocols are used to remove cells and cellular debris
in porcine aortic valve (PAV). However, the removal of antigenic cellular components has …

Tissue engineered (TE) allografts have been successfully applied in pulmonary circulation.
The behavior of TE valves based on decellularized scaffolds in systemic circulation remains …

Objective: Several decellularisation techniques have been developed to produce acellular
matrix scaffolds for the purpose of tissue engineering, mostly comprising (non-) ionic …

We have developed a multistep detergent-enzymatic extraction process (involving hypotonic
and hypertonic solutions, the detergents octyl-phenoxy-polyethoxyethanol and sodium …

Methods: Acellular porcine valve matrix contact and extract cytotoxicity was tested against
porcine fibroblasts and smooth muscle cells (SMC). Porcine cells were incubated with …

Objective: Tissue engineering of heart valves represents a new experimental concept to
improve current modes of therapy in valvular heart disease. Drawbacks of glutaraldehyde …

BACKGROUND: Heart valve bioprostheses for cardiac valve replacement are fabricated by
xeno-or allograft tissues. Decellularization techniques and tissue engineering technologies …