A rapid method for efficient gene replacement in the filamentous fungus Aspergillus nidulans
MK Chaveroche, JM Ghigo, C d'Enfert - Nucleic acids research, 2000 - academic.oup.com
The construction of mutant fungal strains is often limited by the poor efficiency of
homologous recombination in these organisms. Higher recombination efficiencies can be …
homologous recombination in these organisms. Higher recombination efficiencies can be …
Direct and Indirect Gene Replacements in Aspergillus nidulans
BL Miller, KY Miller, WE Timberlake - Molecular and cellular …, 1985 - Taylor & Francis
We performed three sets of experiments to determine whether cloned DNA fragments can be
substituted for homologous regions of the Aspergillus nidulans genome by DNA-mediated …
substituted for homologous regions of the Aspergillus nidulans genome by DNA-mediated …
Cotransformation of Aspergillus nidulans: a tool for replacing fungal genes
K Wernars, T Goosen, BMJ Wennekes, K Swart… - Molecular and General …, 1987 - Springer
When a non-selected DNA sequence was added during the transformation of amdS320
deletion strains of Aspergillus nidulans with a vector containing the wild-type amdS gene the …
deletion strains of Aspergillus nidulans with a vector containing the wild-type amdS gene the …
[引用][C] Sequences important for gene expression in filamentous fungi
DJ Ballance - Yeast, 1986 - Wiley Online Library
The filamentous Ascomycetes Aspergillus nidulans and Neirrospora crassa have been used
extensively for the study of eukaryotic gene organization and regulation using classical …
extensively for the study of eukaryotic gene organization and regulation using classical …
[PDF][PDF] Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans
MJ Daboussi, A Djeballi, C Gerlinger… - Current …, 1989 - researchgate.net
A gene transfer system originally developed for Fusarium oxysporum has been applied to
seven species of filamentous fungi of agricultural and industrial importance. This …
seven species of filamentous fungi of agricultural and industrial importance. This …
Development of a high-frequency transforming vector for Aspergillus nidulans
DJ Ballance, G Turner - Gene, 1985 - Elsevier
The pyr4 gene of Neurospora crassa, which codes for orotidine-5'-phosphate
decarboxylase, is capable of transforming an Aspergillus nidulans pyrG mutant by …
decarboxylase, is capable of transforming an Aspergillus nidulans pyrG mutant by …
Gene targeting is locus dependent in the filamentous fungus Aspergillus nidulans
D Bird, R Bradshaw - Molecular and General Genetics MGG, 1997 - Springer
The effect of altering the conditions of transformation on the efficiency of gene targeting in
filamentous fungi was studied using Aspergillus nidulans as a model organism. The niaD …
filamentous fungi was studied using Aspergillus nidulans as a model organism. The niaD …
Establishing a versatile Golden Gate cloning system for genetic engineering in fungi
M Terfrüchte, B Joehnk, R Fajardo-Somera… - Fungal Genetics and …, 2014 - Elsevier
The corn pathogen Ustilago maydis is a well-studied fungal model organism. Along with a
broad set of experimental tools, versatile strategies for the generation of gene replacement …
broad set of experimental tools, versatile strategies for the generation of gene replacement …
Efficient PCR-based gene targeting with a recyclable marker for Aspergillus nidulans
ML Nielsen, L Albertsen, G Lettier, JB Nielsen… - Fungal Genetics and …, 2006 - Elsevier
The rapid accumulation of genomic sequences from a large number of eukaryotes, including
numerous filamentous fungi, has created a tremendous scientific potential, which can only …
numerous filamentous fungi, has created a tremendous scientific potential, which can only …
Transformation by integration in Aspergillus nidulans
J Tilburn, C Scazzocchio, GG Taylor… - Gene, 1983 - Elsevier
DNA-mediated genetic transformation of Aspergillus nidulans has been achieved by
incubating protoplasts from a strain of A. nidulans carrying a deletion in the acetamidase …
incubating protoplasts from a strain of A. nidulans carrying a deletion in the acetamidase …
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