Tissue browning of in vitro cultures of Scots pine: role of peroxidase and polyphenol oxidase

H Laukkanen, H Häggman… - Physiologia …, 1999 - Wiley Online Library
Callus cultures from shoot tips of mature Scots pine (Pinus sylvestris L.) were characterized
by rapid browning and an inability to regenerate. The peroxidase (POD) and polyphenol …

Changes in cellular structures and enzymatic activities during browning of Scots pine callus derived from mature buds

H Laukkanen, L Rautiainen, E Taulavuori… - Tree …, 2000 - academic.oup.com
Visible browning is a typical feature of callus cultures derived from shoot tips of mature Scots
pine (Pinus sylvestris L.). Because the ability of callus to regenerate is low, we determined …

Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginia pine (Pinus virginiana Mill.)

W Tang, RJ Newton - Plant science, 2004 - Elsevier
Tissue browning seriously decreases in vitro regeneration from callus cultures in some of
woody plants, especially plant regeneration via organogenesis in pine species. Although …

Antioxidants enhance in vitro plant regeneration by inhibiting the accumulation of peroxidase in Virginia pine (Pinus virginiana Mill.)

W Tang, LC Harris, V Outhavong, RJ Newton - Plant cell reports, 2004 - Springer
Plant tissue necrosis and subsequent cell death are usually observed during in vitro
regeneration in conifers, especially in plant regeneration via somatic organogenesis in pine …

Changes of Morphogenic Competence in Mature Pinus sylvestris L. Buds in vitro

U Andersone, G Ievinsh - Annals of botany, 2002 - academic.oup.com
The effects of season and cold storage on morphogenic competence in mature Pinus
sylvestris buds were investigated. Peroxidase and polyphenol oxidase activity were …

Peroxidase Activity, Isoenzymes and Histological Localisation in Sapwood and Heartwood of Scots Pine (Pinus sylvestris L.)

K Fagerstedt, P Saranpää… - Journal of Forest …, 1998 - Taylor & Francis
Peroxidase activity and isoenzymes of fresh wood samples of the third shoot of 12-year old
trees and from the sapwood, transition zone and heartwood of c. 60-year old stems of Scots …

Apoplastic peroxidases and lignification in needles of Norway spruce (Picea abies L.)

A Polle, T Otter, F Seifert - Plant Physiology, 1994 - academic.oup.com
The objective of the present study was to investigate the correlation of soluble apoplastic
peroxidase activity with lignification in needles of field-grown Norway spruce (Picea abies …

Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.)

AM Pirttilä, O Podolich, JJ Koskimäki, E Hohtola… - Plant Cell, Tissue and …, 2008 - Springer
Callus tissues originating from buds of mature Scots pine (Pinus sylvestris L.) trees exhibit
the typical problem of browning, which leads to degeneration and death of the tissues. The …

Correlation between browning, total phenolic content, polyphenol oxidase and several antioxidation enzymes during pistachio tissue culture

P Leng, S Su, F Wei, F Yu, Y Duan - Acta horticulturae, 2009 - actahort.org
A major obstacle to tissue culture of pistachio is the browning of explants, which is usually
attributed to phenolic compounds being oxididied to highly toxic quinone compounds by …

Changes in peroxidase activity associated with cell walls during pine hypocotyl growth

M Sanchez, G Revilla, I Zarra - Annals of Botany, 1995 - academic.oup.com
Abstract Peroxidase active against 2, 2′-azino-bis-[3-ethylbenzthiazoline-6-sulphonic
acid](ABTS) and guaiacol were found in the apoplastic fluid, as well as ionically and …