A chapter on fungi that has been cited more than 10,000 times! Congratulations to Thomas
J. White, Steven B. Lee, Thomas D. Bruns, and John W. Taylor, who pioneered the use of …

We compiled a 5.8 S nuclear ribosomal gene sequence database for animals, plants, and
fungi using both newly generated and GenBank sequences. We demonstrate the utility of …

ABSTRACT rRNA genes are attractive targets for developing PCR assays targeting human
fungal pathogens. Most studies have focused on the 18S rRNA gene, internal transcribed …

The successful extraction of DNA from fungal isolates is essential to the investigation of
fungal genetics at a molecular level (Graham et al. 1994). The ever increasing array of …

The polymerase chain reaction (PCR) is a rapid and sensitive procedure for the in vitro
amplification of specific segments of DNA. The ability to perform primer-directed …

Nuclear ribosomal genes in most eukaryotes are present in multiple copies and often used
for taxonomic and phylogenetic analyses. We comprehensively examined intragenomic …

Fungi are morphologically, ecologically, metabolically, and phylogenetically diverse. They
are known to produce numerous bioactive molecules, which makes them very useful for …

Background Gene conversion is the mechanism proposed to be responsible for the
homogenization of multigene families such as the nuclear ribosomal gene clusters. This …

Nucleotide sequences of the small subunit ribosomal RNA (18S) gene were used to
investigate evolutionary relationships within the Fungi. The inferred tree topologies are in …

In this review, details are given of the amplification process of the polymerase chain reaction
and the application of molecular techniques in determining gene sequences which can be …