Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a
mitochondrial precursor protein, recognized a mitochondrial integral membrane protein …

An engineered precursor protein that sticks in the import site of isolated yeast mitochondria
can be specifically photo-crosslinked to a mitochondrial outer-membrane protein of relative …

MITOCHONDRIA contain a complex machinery for the import of nuclear-encoded proteins1,
2. Receptor proteins exposed on the outer membrane surface are required for the specific …

The mitochondrial import receptors MOM19 and MOM72 form a complex with two other
proteins of the mitochondrial outer membrane, MOM38 and MOM22. This receptor complex …

WE have reconstituted the initial steps of mitochondrial protein import with a purified
precursor protein, a purified, ATP-depend-ent, cytosolic chaperone selective for …

THE principle is simple yet elegant. An anti-receptor antibody can be produced without
having to use the receptor as an antigen. The technique is based on the fact that the binding …

We have identified a 19 kd protein of the mitochondrial outer membrane (MOM19).
Monospecific IgG and Fab fragments directed against MOM19 inhibit import of precursor …

The gene encoding ISP42, an integral outer-membrane protein located at the yeast
mitochondrial protein import site was cloned, sequenced and modified. Yeast cells depleted …

Publisher Summary This chapter describes the standard procedures for in vitro synthesis
and import of precursor proteins. Various translocation intermediates can be generated by …

The targeting of proteins to mitochondria involves the recognition of the precursor proteins
by receptors on the mitochondrial surface followed by insertion of the precursors into the …