PhenoGMM: Gaussian mixture modeling of cytometry data quantifies changes in microbial community structure

P Rubbens, R Props, FM Kerckhof, N Boon… - MSphere, 2021 - Am Soc Microbiol
Microbial flow cytometry can rapidly characterize the status of microbial communities. Upon
measurement, large amounts of quantitative single-cell data are generated, which need to …

Predicting the presence and abundance of bacterial taxa in environmental communities through flow cytometric fingerprinting

J Heyse, F Schattenberg, P Rubbens, S Müller… - Msystems, 2021 - Am Soc Microbiol
Microbiome management research and applications rely on temporally resolved
measurements of community composition. Current technologies to assess community …

flowEMMi: an automated model-based clustering tool for microbial cytometric data

J Ludwig, CH Zu Siederdissen, Z Liu, PF Stadler… - BMC …, 2019 - Springer
Background Flow cytometry (FCM) is a powerful single-cell based measurement method to
ascertain multidimensional optical properties of millions of cells. FCM is widely used in …

Application of flow cytometry for rapid, high-throughput, multiparametric analysis of environmental microbiomes

M Priyadarsini, J Kushwaha, KP Pandey, J Rani… - Journal of …, 2023 - Elsevier
Quantification of the abundance and understanding of the dynamics of the microbial
communities is essential to establish a basis for microbiome characterization. The …

Computational analysis of microbial flow cytometry data

P Rubbens, R Props - MSystems, 2021 - Am Soc Microbiol
Flow cytometry is an important technology for the study of microbial communities. It grants
the ability to rapidly generate phenotypic single-cell data that are both quantitative …

Development of an automated online flow cytometry method to quantify cell density and fingerprint bacterial communities

J López-Gálvez, K Schiessl, MD Besmer, C Bruckmann… - Cells, 2023 - mdpi.com
Cell density is an important factor in all microbiome research, where interactions are of
interest. It is also the most important parameter for the operation and control of most …

Bacterial mock communities as standards for reproducible cytometric microbiome analysis

N Cichocki, T Hübschmann, F Schattenberg… - Nature …, 2020 - nature.com
Flow cytometry has recently established itself as a tool to track short-term dynamics in
microbial community assembly and link those dynamics with ecological parameters …

Measuring the biodiversity of microbial communities by flow cytometry

R Props, P Monsieurs, M Mysara… - Methods in Ecology …, 2016 - Wiley Online Library
Measuring the microbial diversity in natural and engineered environments is important for
ecosystem characterization, ecosystem monitoring and hypothesis testing. Although the …

Cytometric fingerprints: evaluation of new tools for analyzing microbial community dynamics

C Koch, F Harnisch, U Schröder, S Müller - Frontiers in microbiology, 2014 - frontiersin.org
Optical characteristics of individual bacterial cells of natural communities can be measured
with flow cytometry (FCM) in high throughput. The resulting data are visualized in cytometric …

CHIC—an automated approach for the detection of dynamic variations in complex microbial communities

C Koch, I Fetzer, H Harms, S Müller - Cytometry Part A, 2013 - Wiley Online Library
Altering environmental conditions change structures of microbial communities. These effects
have an impact on the single‐cell level and can be sensitively detected using community …