The cloning, overexpression and characterization of a cold‐adapted DNA ligase from the
Antarctic sea water bacterium Pseudoalteromonas haloplanktis are described. Protein …

In this paper we describe the cloning and sequence analysis of a gene encoding DNA
ligase (Lig; EC 6.5. 1.2) from the thermophilic bacterium Rhodothermus marinus (Rm). We …

Limited proteolysis of the NAD+-dependent DNA ligase from Bacillus stearothermophilus
with thermolysin results in two fragments which were resistant to further proteolysis. These …

Psychrophiles, host of permanently cold habitats, display metabolic fluxes comparable to
those exhibited by mesophilic organisms at moderate temperatures. These organisms have …

DNA ligases are important enzymes required for cellular processes such as DNA replication,
recombination, and repair. NAD+-dependent DNA ligases are essentially restricted to …

An NAD+-dependent DNA ligase from the hyperthermophilic bacterium Aquifex aeolicus
was cloned, expressed in Escherichia coli and purified to homogeneity. The enzyme is most …

DNA ligases play a pivotal role in DNA replication, repair and recombination. Reactions
catalyzed by DNA ligases consist of three steps: adenylation of the ligase in the presence of …

In the last few years, an increased attention has been focused on NAD+-dependent DNA
ligases. This is mostly due to their potential use as antibiotic targets, because effective …

DNA ligases operating at low temperatures have potential advantages for use in
biotechnological applications. For this reason, we have characterized the temperature …

NAD+-dependent DNA ligases are essential enzymes in bacteria, with the most widely
studied of this class of enzymes being LigA from Escherichia coli. NAD+-dependent DNA …