Cell sensitivity assays: the MTT assay

J Van Meerloo, GJL Kaspers, J Cloos - Cancer cell culture: methods and …, 2011 - Springer
Abstract The MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay is
based on the conversion of MTT into formazan crystals by living cells, which determines …

MTT assays

R Supino - In vitro toxicity testing protocols, 1995 - Springer
The MTT calorimetric assay determines the ability of viable cells to convert a soluble
tetrazolium salt [3–(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide](MTT) into an …

Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability

PW Sylvester - Drug Design and Discovery: Methods and Protocols, 2011 - Springer
The MTT colorimetric assay is an established method of determining viable cell number in
proliferation and cytotoxicity studies. This assay is based on the cleavage of the yellow …

[PDF][PDF] Interference in MTT cell viability assay in activated macrophage cell line

M Pozzolini, S Scarfı̀, U Benatti… - Analytical …, 2003 - zjubiolab.zju.edu.cn
The MTT1 (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay is a simple
colorimetric method to measure cytotoxicity, proliferation, or cell viability first developed by …

Analysis of cell viability by the MTT assay

P Kumar, A Nagarajan… - Cold spring harbor …, 2018 - cshprotocols.cshlp.org
Among viability assays that depend on the conversion of substrate to chromogenic product
by live cells, the MTT assay is still among one of the most versatile and popular assays. The …

Validation of the MTT dye assay for enumeration of cells in proliferative and antiproliferative assays.

BE Loveland, TG Johns, IR Mackay… - Biochemistry …, 1992 - europepmc.org
Increasing use is being made of colorimetric assays to quantitate viable cells, eg, the cellular
reduction of the tetrazolium salt, MTT, to formazan by mitochondrial succinate …

General cytotoxicity assessment by means of the MTT assay

L Tolosa, MT Donato, MJ Gómez-Lechón - Protocols in in vitro hepatocyte …, 2015 - Springer
Cytotoxicity assays were among the first in vitro bioassay methods used to predict toxicity of
substances to various tissues. In vitro cytotoxicity testing provides a crucial means for safety …

Characterization of the cellular reduction of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and …

MV Berridge, AS Tan - Archives of biochemistry and biophysics, 1993 - Elsevier
The MTT assay, which is widely used to measure cell proliferation and to screen for
anticancer drugs, is based on reduction of the tetrazolium salt, MTT (3-(4, 5-dimethylthiazol …

The MTT tetrazolium salt assay scrutinized: How to use this assay reliably to measure metabolie activity of cell cultures in vitro for the assessment of growth …

AM Sieuwerts, JGM Klijn, HA Peters, JA Foekens - 1995 - degruyter.com
The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliumbromide (MTT) assay is widely
used for in vitro measurement of the metabolic viability of cell cultures subjected to different …

Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing

J Carmichael, WG DeGraff, AF Gazdar, JD Minna… - Cancer research, 1987 - AACR
Drug sensitivity assays were performed using a variation of a colorimetric [3-(4, 5-
dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT)] assay on V79, CHO-AuxB1 …