Phase transitions and size scaling of membrane-less organelles

CP Brangwynne - Journal of Cell Biology, 2013 - rupress.org
The coordinated growth of cells and their organelles is a fundamental and poorly
understood problem, with implications for processes ranging from embryonic development …

Multiple modes of protein–protein interactions promote RNP granule assembly

T Mittag, R Parker - Journal of molecular biology, 2018 - Elsevier
Eukaryotic cells are known to contain a wide variety of RNA–protein assemblies, collectively
referred to as RNP granules. RNP granules form from a combination of RNA–RNA, protein …

Independent active and thermodynamic processes govern the nucleolus assembly in vivo

H Falahati, E Wieschaus - Proceedings of the National …, 2017 - National Acad Sciences
Membraneless organelles play a central role in the organization of protoplasm by
concentrating macromolecules, which allows efficient cellular processes. Recent studies …

Nucleation by rRNA dictates the precision of nucleolus assembly

H Falahati, B Pelham-Webb, S Blythe, E Wieschaus - Current Biology, 2016 - cell.com
Membrane-less organelles are intracellular compartments specialized to carry out specific
cellular functions. There is growing evidence supporting the possibility that such organelles …

Inverse size scaling of the nucleolus by a concentration-dependent phase transition

SC Weber, CP Brangwynne - Current Biology, 2015 - cell.com
Just as organ size typically increases with body size, the size of intracellular structures
changes as cells grow and divide. Indeed, many organelles, such as the nucleus [1, 2] …

Soft active aggregates: mechanics, dynamics and self-assembly of liquid-like intracellular protein bodies

CP Brangwynne - Soft Matter, 2011 - pubs.rsc.org
Intracellular bodies consisting of dynamic aggregates of concentrated proteins and often
RNA are a ubiquitous feature of the cytoplasm and nucleus of living cells. Dozens of different …

Beyond stereospecificity: liquids and mesoscale organization of cytoplasm

AA Hyman, CP Brangwynne - Developmental cell, 2011 - cell.com
The cytoplasm is not a homogenous solution but instead consists of large dynamic
assemblies that arise from transient molecular interactions. Some of these structures have …

Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles

JE Lee, PI Cathey, H Wu, R Parker, GK Voeltz - Science, 2020 - science.org
INTRODUCTION The cytoplasm contains an unconventional class of organelles that
concentrate specific factors and resources without a limiting membrane. These …

Phase separation in biology; functional organization of a higher order

DM Mitrea, RW Kriwacki - Cell Communication and Signaling, 2016 - Springer
Inside eukaryotic cells, macromolecules are partitioned into membrane-bounded
compartments and, within these, some are further organized into non-membrane-bounded …

Organelle growth control through limiting pools of cytoplasmic components

NW Goehring, AA Hyman - Current Biology, 2012 - cell.com
The critical importance of controlling the size and number of intracellular organelles has led
to a variety of mechanisms for regulating the formation and growth of cellular structures. In …