Size exclusion chromatography: a simple and reliable method for exosome purification

R Lobb, A Möller - Extracellular Vesicles: Methods and Protocols, 2017 - Springer
Exosomes were originally described 29 years ago as a mechanism for the removal of
redundant molecules from reticulocytes, nothing but a process of removing cellular trash. It is …

Exosome isolation by ultracentrifugation and precipitation and techniques for downstream analyses

C Coughlan, KD Bruce, O Burgy… - Current protocols in …, 2020 - Wiley Online Library
Abstract Exosomes are 50‐to 150‐nm‐diameter extracellular vesicles secreted by all
mammalian cells except mature red blood cells and contribute to diverse physiological and …

Isolation of exosomes from blood plasma: qualitative and quantitative comparison of ultracentrifugation and size exclusion chromatography methods

T Baranyai, K Herczeg, Z Onódi, I Voszka, K Módos… - PloS one, 2015 - journals.plos.org
Background Exosomes are emerging targets for biomedical research. However, suitable
methods for the isolation of blood plasma-derived exosomes without impurities have not yet …

[PDF][PDF] Development of a rinsing separation method for exosome isolation and comparison to conventional methods.

H Cheng, H Fang, RD Xu, MQ Fu… - European Review for …, 2019 - europeanreview.org
OBJECTIVE: Exosomes contain valuable biomarkers for many diseases. Tragically,
standardized isolation methods and subsequent characterization criteria for exosomes …

Optimized exosome isolation protocol for cell culture supernatant and human plasma

RJ Lobb, M Becker, S Wen Wen… - Journal of …, 2015 - Taylor & Francis
Extracellular vesicles represent a rich source of novel biomarkers in the diagnosis and
prognosis of disease. However, there is currently limited information elucidating the most …

Benchtop isolation and characterization of functional exosomes by sequential filtration

ML Heinemann, M Ilmer, LP Silva, DH Hawke… - … of Chromatography A, 2014 - Elsevier
Early and minimally invasive detection of malignant events or other pathologies is of utmost
importance in the pursuit of improved patient care and outcomes. Recent evidence indicates …

A comparative study of serum exosome isolation using differential ultracentrifugation and three commercial reagents

I Helwa, J Cai, MD Drewry, A Zimmerman, MB Dinkins… - PloS one, 2017 - journals.plos.org
Exosomes play a role in cell-to-cell signaling and serve as possible biomarkers. Isolating
exosomes with reliable quality and substantial concentration is a major challenge. Our …

[PDF][PDF] Exosome enrichment by ultracentrifugation and size exclusion chromatography

YQ Koh, FB Almughlliq, K Vaswani… - Front Biosci …, 2018 - article.imrpress.com
Exosomes are a subset of extracellular vesicles (EVs) that have important roles in
intercellular communication. They contain and carry bioactive molecules within their …

Isolation of biologically-active exosomes from human plasma

L Muller, CS Hong, DB Stolz, SC Watkins… - Journal of …, 2014 - Elsevier
Abstract Effects of exosomes present in human plasma on immune cells have not been
examined in detail. Immunological studies with plasma-derived exosomes require their …

A novel “salting-out” procedure for the isolation of tumor-derived exosomes

Z Brownlee, KD Lynn, PE Thorpe, AJ Schroit - Journal of immunological …, 2014 - Elsevier
The last decade has seen an exponential growth in the number of exosome-related
publications. Although many of these studies have used exosomes from biological fluids …