Realistic quantitative models require data from many laboratories. Therefore,
standardization of experimental systems and assay conditions is crucial. Moreover …

Regulation of fermentative capacity was studied in chemostat cultures of two
Saccharomyces cerevisiae strains: the laboratory strain CEN. PK113-7D and the industrial …

A decade ago, a team of biochemists including two of us, modeled yeast glycolysis and
showed that one of the most studied biochemical pathways could not be quite understood in …

To select a Saccharomyces cerevisiae reference strain amenable to experimental
techniques used in (molecular) genetic, physiological and biochemical engineering …

Realistic mathematical models of biochemical pathways require kinetic data that have been
measured under physiological conditions. However, until recently most enzyme-kinetic data …

Aerobic chemostat cultures of Saccharomyces cerevisiae were performed under carbon-,
nitrogen-, and dual carbon-and nitrogen-limiting conditions. The glucose concentration was …

Values of enzyme kinetic parameters are a key requisite for the kinetic modelling of
biochemical systems. For most kinetic parameters, however, not even an order of magnitude …

The field of systems biology is often held back by difficulties in obtaining comprehensive,
high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to …

Growth, substrate consumption, metabolite formation, biomass composition and respiratory
parameters of Kluyveromyces marxianus ATCC 26548 were determined during aerobic …

An integrated approach is used to develop a rapid sampling strategy for the quantitative
analysis of in vivo kinetic behavior based on measured concentrations of intracellular …