Combinatorial peptide ligand libraries for urine proteome analysis: investigation of different elution systems

G Candiano, V Dimuccio, M Bruschi, L Santucci… - …, 2009 - Wiley Online Library
Proteome treatments with peptide libraries in view of reducing high‐abundance proteins and
increasing the concentration of rare species involve the adsorption on solid‐phase material …

Plucking, pillaging and plundering proteomes with combinatorial peptide ligand libraries

PG Righetti, E Boschetti, A Zanella, E Fasoli… - … of Chromatography A, 2010 - Elsevier
Recent developments in the technique of combinatorial peptide ligand libraries, for
enhancing the visibility of the low-abundance proteome, are reviewed here. Novel en bloc …

Combinatorial peptide ligand libraries for the analysis of low‐expression proteins: Validation for normal urine and definition of a first protein MAP

L Santucci, G Candiano, M Bruschi, C D'Ambrosio… - …, 2012 - Wiley Online Library
In this review, we report the evolution on experimental conditions for the analysis of normal
urine based on combinatorial peptide ligand library (CPLL) treatment and successive 2‐DE …

“Cheek-to-cheek” urinary proteome profiling via combinatorial peptide ligand libraries: a novel, unexpected elution system

G Candiano, L Santucci, M Bruschi, A Petretto… - Journal of …, 2012 - Elsevier
A new method is here reported for facile elution of the human urinary proteome after being
captured with combinatorial peptide ligand libraries (CPLL, ProteoMiner). It consists in …

Combinatorial peptide ligand libraries as a “Trojan Horse” in deep discovery proteomics

PG Righetti, G Candiano, A Citterio… - Analytical …, 2015 - ACS Publications
It is of common and widespread knowledge that proteomes are populated by not only a very
large number of proteins but more importantly by a few highly concentrated proteins and a …

The art of observing rare protein species in proteomes with peptide ligand libraries

E Boschetti, PG Righetti - Proteomics, 2009 - Wiley Online Library
The present review deals with the use of combinatorial ligand libraries, composed by
hexapeptides, in the capture and concentration of the low‐abundance proteome. This …

The proteome buccaneers: how to unearth your treasure chest via combinatorial peptide ligand libraries

PG Righetti, E Boschetti, AV Kravchuk… - Expert review of …, 2010 - Taylor & Francis
The latest advances in combinatorial peptide ligand libraries, with their unique performance
in discovering low-abundance species in proteomes, are reviewed here. Explanations of …

Combinatorial peptide libraries to overcome the classical affinity-enrichment methods in proteomics

PG Righetti, E Boschetti - Amino Acids, 2013 - Springer
The enrichment of targeted low-abundance proteins is possible by affinity adsorption using
selected sorbents. Different categories of very dilute proteins are present in most of …

Interaction among proteins and peptide libraries in proteome analysis: pH involvement for a larger capture of species

E Fasoli, A Farinazzo, CJ Sun, AV Kravchuk… - Journal of …, 2010 - Elsevier
When capturing proteins via combinatorial peptide ligand libraries, a method well known for
drastically reducing the concentration of high-abundance proteins and substantially …

[PDF][PDF] Protein sample treatment with peptide ligand library: coverage and consistency

L Li, C Sun, S Freeby, D Yee, S Kieffer-Jaquinod… - J Proteomics …, 2009 - Citeseer
Low-abundance protein detection in biological samples is one of the main challenges in
proteomics investigations. One approach that makes the detection of rare species possible …