PROTOCOL 6.32 Detection of cytomatrix proteins by immunogold embedment-free electron microscopy
R Gniadecki, B Gajkowska - Cell Biology Protocols, 2006 - Wiley Online Library
The simple view of the cell as the membrane sack containing organelles floating in the
amorphous cytoplasm has over the last 30 years been replaced by a concept of a …
amorphous cytoplasm has over the last 30 years been replaced by a concept of a …
Electron Microscopy of Extracted Cytoskeletons: Negative Staining, Cryoelectron Microscopy, and Correlation with Light Microscopy
GP Resch, JV Small, KN Goldie - Cell Biology, 2006 - Elsevier
Publisher Summary This chapter describes the application of negative staining and cryo-EM
to the cytoskeleton research, highlighting the feasibility of correlating information on the …
to the cytoskeleton research, highlighting the feasibility of correlating information on the …
Immunolocalization in three dimensions: immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections.
JA Nickerson, G Krockmalnic… - Proceedings of the …, 1990 - National Acad Sciences
We describe two methods for staining resinless thin sections with antibodies and gold-
conjugated second antibodies. Immunolocalization of specific proteins is a powerful tool for …
conjugated second antibodies. Immunolocalization of specific proteins is a powerful tool for …
Improved representation of cell surface structures by freeze substitution and backscattered electron imaging.
P Walther, J Hentschel - Scanning microscopy. Supplement, 1989 - europepmc.org
A surface preparation method for the SEM based on cryofixation is presented suitable for the
demonstration of membrane particles on whole cells. LLC-PK1 cells (a renal epithelial cell …
demonstration of membrane particles on whole cells. LLC-PK1 cells (a renal epithelial cell …
Electron microscopy in cell biology
G Griffiths - Reviews in Cell Biology and Molecular Medicine, 2006 - Wiley Online Library
Most of the organelles discovered after 1945 were first seen by electron microscopy (EM) in
thin sections of resin‐embedded cells. Since that time, the technical advances in EM …
thin sections of resin‐embedded cells. Since that time, the technical advances in EM …
Study of vitrified, unstained frozen tissue sections by cryoimmunoelectron microscopy
I Sabanay, T Arad, S Weiner… - Journal of cell …, 1991 - journals.biologists.com
We describe the development and application of a novel approach to high-resolution
ultrastructural analysis of cells and tissues. It is based on the preparation of ultrathin frozen …
ultrastructural analysis of cells and tissues. It is based on the preparation of ultrathin frozen …
Ultrathin cryosections in the plane of cell monolayers: evaluation of their potential for antibody localization studies of the cytoskeleton
G Langanger, J De Mey - Journal of electron microscopy …, 1988 - Wiley Online Library
In spite of the fact that the preembedding method is satisfactory for the ultrastructural
localization of cytoskeletal proteins, there is a need for a localization method that retains the …
localization of cytoskeletal proteins, there is a need for a localization method that retains the …
Cryo-EM and single particles
L Wang, FJ Sigworth - Physiology, 2006 - journals.physiology.org
Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron
microscope. It was originally developed to determine membrane protein structures from two …
microscope. It was originally developed to determine membrane protein structures from two …
Immunoelectron microscopy
EJ van Tuinen - Yeast Protocols: Methods in Cell and Molecular …, 1996 - Springer
The immunolocalization of proteins on sections of biological materials that have been
prepared for electron microscopy, requires the preservation of both antigenic and …
prepared for electron microscopy, requires the preservation of both antigenic and …