Microplate-based high throughput screening procedure for the isolation of lipid-rich marine microalgae

H Pereira, L Barreira, A Mozes, C Florindo… - Biotechnology for …, 2011 - Springer
We describe a new selection method based on BODIPY (4, 4-difluoro-1, 3, 5, 7-tetramethyl-4-
bora-3a, 4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and …

Enhancement of BODIPY505/515 lipid fluorescence method for applications in biofuel-directed microalgae production

L Brennan, AB Fernández, AS Mostaert… - Journal of microbiological …, 2012 - Elsevier
This paper describes a microalgal cell lipid fluorescence enhancement method using
BODIPY505/515, which can be used to screen for lipids in wild-type microalgae and to …

Digital quantification and selection of high-lipid-producing microalgae through a lateral dielectrophoresis-based microfluidic platform

SI Han, HS Kim, KH Han, A Han - Lab on a Chip, 2019 - pubs.rsc.org
Microalgae are promising alternatives to petroleum as renewable biofuel sources, however
not sufficiently economically competitive yet. Here, a label-free lateral dielectrophoresis …

Isolation and fatty acid profile of selected microalgae strains from the Red Sea for biofuel production

H Pereira, L Barreira, L Custódio, S Alrokayan… - Energies, 2013 - mdpi.com
The isolation of lipid-rich autochthonous strains of microalgae is a crucial stage for the
development of a microalgae-based biofuel production plant, as these microalgae already …

Bioprospecting for hyper-lipid producing microalgal strains for sustainable biofuel production

T Mutanda, D Ramesh, S Karthikeyan, S Kumari… - Bioresource …, 2011 - Elsevier
Global petroleum reserves are shrinking at a fast pace, increasing the demand for alternate
fuels. Microalgae have the ability to grow rapidly, and synthesize and accumulate large …

An integrated microfluidic device for the high-throughput screening of microalgal cell culture conditions that induce high growth rate and lipid content

S Bae, CW Kim, JS Choi, JW Yang, TS Seo - Analytical and bioanalytical …, 2013 - Springer
This study describes the development of a microfluidic device for the high-throughput
screening of culture conditions, such as the optimum sodium acetate concentration for …

Isolation of high-lipid content strains of the marine microalga Tetraselmis suecica for biodiesel production by flow cytometry and single-cell sorting

MF Montero, M Aristizábal, G García Reina - Journal of Applied Phycology, 2011 - Springer
Biodiesel from algae is considered an alternative for a third generation of biofuels. However,
most microalgae are not lipogenic during fast growth periods, but high-lipid content occurs at …

In situ analysis of heterogeneity in the lipid content of single green microalgae in alginate hydrogel microcapsules

DH Lee, CY Bae, JI Han, JK Park - Analytical Chemistry, 2013 - ACS Publications
Microalgae, a group of microorganisms that grow using sunlight as the sole energy source
and carbon dioxide as an only carbon source, have been considered as a feedstock of …

Oil production towards biofuel from autotrophic microalgae semicontinuous cultivations monitorized by flow cytometry

TL Da Silva, A Reis, R Medeiros, AC Oliveira… - Applied biochemistry …, 2009 - Springer
Two microalgae species (Scenedesmus obliquus and Neochloris oleoabundans) were
cultivated in closed sleeve photobioreactors in order to select the best oil producer for further …

Enhanced lipid production in Nannochloropsis sp. using fluorescence‐activated cell sorting

THIT YEN DOAN, JP Obbard - Gcb Bioenergy, 2011 - Wiley Online Library
To advance the utilization of microalgae as a viable feedstock for biodiesel production, the
intracellular lipid content of three strains of the marine microalgae Nannochloropsis sp. was …