Recent studies in our laboratory have indicated that bovine embryos only use a small
amount of the nutrients available to them in culture. Our objective was to evaluate the …

To elucidate the effect of nutrient substrates on embryo development, in vitro fertilized
bovine one-cell embryos were cultured in a medium similar to synthetic oviduct fluid (SOF) …

Due to the complicated media used for culturing bovine embryos, most of the nutrient
requirements are unknown. Recently, we developed a simple, serum-free medium (CR1) …

The refinement of embryo culture media is essential in improving embryo viability and in
vitro production efficiency. Our previous work demonstrated that the nutrients …

The present study compared the developmental potential and uptake of nutrients by
embryos from pre‐pubertal and adult cows. Oocytes retrieved from ovaries of 5 to 7 month …

Two major considerations governing choice of culture system for in vitro production of
bovine and other mammalian embryos are the efficiency of blastocyst production and the …

Reproducing the environment to which the embryo is naturally exposed may be an
alternative to improve viability of embryos produced in vitro. In the first part of this work, we …

Effects of amino acids on early bovine embryo development in vitro were examined using a
chemically-defined, protein-free culture medium. Bovine embryos produced in vitro were …

Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …

Amino acids have been shown to improve the development of in vitro fertilised bovine 1-cell
embryos to morulae and blastocysts, and increase total cell numbers at the blastocyst stage …