Cloning by somatic cell nuclear transfer is critically dependent upon early events that occur
immediately after nuclear transfer, and possibly additional events that occur in the cleaving …

The successful production of viable progeny following adult somatic cell nuclear transfer
(cloning) provides exciting new opportunities for basic research for investigating early …

The effect of simple and sequential embryo culture media on the preimplantation
development of mouse nuclear transfer (NT) embryos reconstructed with cumulus cell nuclei …

ABREAKTHROUGH was made by Wilmut and his colleagues in 1996. A sheep named Dolly
was successfully cloned from an adult somatic cell (Wilmut et al., 1997). In this experiment …

In this report we demonstrate the successful in vitro culture of fertilised embryos from 1-cell
to blastocyst stage, albeit in a strain-dependent fashion. We report procedures for the …

Cloning by somatic cell nuclear transfer (SCNT) in mammals has revealed the remarkable
ability of an oocyte to reprogram somatic cell nuclei and induce them to recapitulate the …

Cloning by the transfer of adult somatic cell nuclei to oocytes has produced viable offspring
in a variety of mammalian species. The technology is still in its initial stages of development …

Cloning by somatic cell nuclear transfer requires silencing of the donor cell gene expression
program and the initiation of the embryonic gene expression program (nuclear …

Enucleated mouse zygotes receiving eight-cell nuclei rarely develop beyond the two-cell
stage, whereas enucleated two-cell embryos with eight-cell nuclei develop to blastocysts …

TWO TECHNICAL FACTORS appear to be critical to the success of rabbit cloning: timing of
activation of the recipient oocyte (Collas and Robl, 1990) and synchronization of the …