The hha gene modulates haemolysin expression in Escherichia coli

JM Nieto, M Carmona, S Bolland, Y Jubete… - Molecular …, 1991 - Wiley Online Library
A mutation in the hha allele results in a large increase in the production of intracellular as
well as extracellular haemolysin in Escherichia coli cells harbouring the haemolytic …

Characterization of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli

M Vogel, J Hess, I Then, A Juarez, W Goebel - Molecular and General …, 1988 - Springer
A sequence (hly R) of about 600 bp which enhances the expression of hemolysin (HlyA)
more than 50-fold was identified in the plasmid pHly152-specific hemolysin (hly) …

Chromosomal Mutations That Increase the Production of a Plasmid-encoded Haemolysin in Escherichia coli

N Godessart, FJ Munoa, M Regue… - Microbiology, 1988 - microbiologyresearch.org
Transposon mutagenesis was used to isolate two Escherichia coli mutants which express
very large amounts of haemolysin when carrying the multicopy plasmid pANN202-312. E …

Regulation of haemolysin synthesis in E. coli determined by HLY genes of human origin

JM Nicaud, N Mackman, L Gray, IB Holland - Molecular and General …, 1985 - Springer
We have previously reported the secretion of a 107K polypeptide into the medium from a
haemolytic E. coli K12 strain (Mackman and Holland 1984a). In addition, we demonstrated …

Expression and regulation of the plasmid-encoded hemolysin determinant of Escherichia coli

A Juarez, C Hughes, M Vogel, W Goebel - Molecular and General …, 1984 - Springer
As a first approach towards studying the regulation of hemolysin synthesis in Escherichia
coli, we have fused lacZ into the four hly genes (hlyC, hlyA, hlyB a and hlyB b) using the Mu …

Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli

W Goebel, J Hedgpeth - Journal of bacteriology, 1982 - Am Soc Microbiol
We cloned the DNA containing the Escherichia coli hemolysin determinant on a small, high-
copy plasmid. We generated plasmids containing fragments of this DNA and used them …

Transcriptional organization of the Escherichia coli hemolysin genes

RA Welch, S Pellett - Journal of bacteriology, 1988 - Am Soc Microbiol
The transcriptional organization of the Escherichia coli hemolysin genes (hlyCABD)
encoded by pSF4000 was examined. The use of different hemolysin gene-specific …

Nucleotide sequence of a plasmid-encoded hemolysin determinant and its comparison with a corresponding chromosomal hemolysin sequence

J Hess, W Wels, M Vogel… - FEMS Microbiology …, 1986 - academic.oup.com
The complete sequence of the plasmid pHly152-encoded hemolysin (hly) determinant of
Escherichia coli is presented and compared with a recently sequenced chromosomal hly …

Functional characterization of a cloned haemolysin determinant from E. coli of human origin, encoding information for the secretion of a 107K polypeptide

N Mackman, IB Holland - Molecular and General Genetics MGG, 1984 - Springer
We have recently reported the secretion of a 107K polypeptide by an E. coli strain containing
the haemolytic plasmid pHly167 (Mackman and Holland 1984). In this paper we show that a …

Purification of α-hemolysin from an overproducing E. coli strain

MI Gonzalez-Carrero, JC Zabala, F De la Cruz… - Molecular and General …, 1985 - Springer
The genetic determinant of the α-hemolysin encoded by plasmid pHly152 has been cloned
in both orientations in plasmid pBR322 giving rise to plasmids pSU157 and pSU158. E. coli …