Development and evaluation of a real-time polymerase chain reaction test for the detection of Theileria parva infections in Cape buffalo (Syncerus caffer) and cattle

KP Sibeko, MC Oosthuizen, NE Collins, D Geysen… - Veterinary …, 2008 - Elsevier
Corridor disease, caused by the tick-borne protozoan parasite Theileria parva, is a
controlled disease in South Africa. The Cape buffalo is the reservoir host and uninfected …

Evaluation of a TaqMan real-time PCR for the detection of Theileria parva in buffalo and cattle

N Papli, O Landt, C Fleischer, JO Koekemoer… - Veterinary …, 2011 - Elsevier
A real-time PCR assay based on TaqMan probe chemistry was developed for the detection
of Theileria parva DNA in blood samples. It uses a Theileria genus-specific PCR primer set …

The persistence of Theileria parva infection in cattle immunized using two stocks which differ in their ability to induce a carrier state: analysis using a novel blood spot …

RA Skilton, RP Bishop, JM Katende, S Mwaura… - Parasitology, 2002 - cambridge.org
An improved Theileria parva DNA detection assay based on the polymerase chain reaction
(PCR) using primers derived from the 104 kDa antigen (p104) gene was developed to detect …

A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals

DO Odongo, JD Sunter, HK Kiara, RA Skilton… - Parasitology …, 2010 - Springer
Abstract Theileria parva causes East Coast fever, an economically important disease of
cattle in sub-Saharan Africa. We describe a nested polymerase chain reaction (nPCR) assay …

An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule

J Katende, S Morzaria, P Toye, R Skilton, V Nene… - Parasitology …, 1998 - Springer
Field and experimental bovine infection sera were used in immunoblots of sporozoite and
schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite …

Development of a multiplex PCR assay for detection and discrimination of Theileria annulata and Theileria sergenti in cattle

L Junlong, Y Li, A Liu, G Guan, J Xie, H Yin, J Luo - Parasitology research, 2015 - Springer
Aim to construct a simple and efficient diagnostic assay for Theileria annulata and Theileria
sergenti, a multiplex polymerase chain reaction (PCR) method was developed in this study …

The Hybrid II assay: a sensitive and specific real-time hybridization assay for the diagnosis of Theileria parva infection in Cape buffalo (Syncerus caffer) and cattle

R Pienaar, FT Potgieter, AA Latif, OMM Thekisoe… - Parasitology, 2011 - cambridge.org
Corridor disease is an acute, fatal disease of cattle caused by buffalo-adapted Theileria
parva. This is a nationally controlled disease in South Africa and strict control measures …

[PDF][PDF] Qualitative and quantitative assessment of Theileria annulata in cattle and buffaloes Polymerase Chain Reaction.

VR Kundave, AK Patel, PV Patel, JJ Hasnani, CG Joshi - 2014 - msptm.org
Bovine tropical theileriosis caused by Theileria annulata is a tick-borne disease associated
with high morbidity and mortality in the livestock. The conventional method of diagnosis is by …

Detection of a carrier state in Theileria parva-infected cattle by the polymerase chain reaction

R Bishop, B Sohanpal, DP Kariuki, AS Young, V Nene… - Parasitology, 1992 - cambridge.org
Two sets of oligonucleotide primers, one derived from a repetitive sequence and the other
from the gene encoding a 67 kDa sporozoite antigen of Theileria parva, were used to …

Mixed Theileria infections in free-ranging buffalo herds: implications for diagnosing Theileria parva infections in Cape buffalo (Syncerus caffer)

R Pienaar, FT Potgieter, AA Latif, OMM Thekisoe… - Parasitology, 2011 - cambridge.org
Buffalo-adapted Theileria parva causes Corridor disease in cattle. Strict control measures
therefore apply to the movement of buffalo in South Africa and include mandatory testing of …