Protonation‐mediated structural flexibility in the F conjugation regulatory protein, TraM
TraM is essential for F plasmid‐mediated bacterial conjugation, where it binds to the plasmid
DNA near the origin of transfer, and recognizes a component of the transmembrane DNA …
DNA near the origin of transfer, and recognizes a component of the transmembrane DNA …
Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM
The conjugative transfer of F-like plasmids such as F, R1, R100 and pED208, between
bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers …
bacterial cells requires TraM, a plasmid-encoded DNA-binding protein. TraM tetramers …
Mechanistic basis of plasmid-specific DNA binding of the F plasmid regulatory protein, TraM
The conjugative transfer of bacterial F plasmids relies on TraM, a plasmid-encoded protein
that recognizes multiple DNA sites to recruit the plasmid to the conjugative pore. In spite of …
that recognizes multiple DNA sites to recruit the plasmid to the conjugative pore. In spite of …
Mutational analysis of TraM correlates oligomerization and DNA binding with autoregulation and conjugative DNA transfer
J Lu, W Zhao, LS Frost - Journal of Biological Chemistry, 2004 - ASBMB
F plasmid TraM, an autoregulatory homotetramer, is essential for F plasmid bacterial
conjugative transfer, one of the major mechanisms for horizontal gene dissemination. TraM …
conjugative transfer, one of the major mechanisms for horizontal gene dissemination. TraM …
Mutations in the C-terminal region of TraM provide evidence for in vivo TraM-TraD interactions during F-plasmid conjugation
J Lu, LS Frost - Journal of bacteriology, 2005 - Am Soc Microbiol
Conjugation is a major mechanism for disseminating genetic information in bacterial
populations, but the signal that triggers it is poorly understood in gram-negative bacteria. F …
populations, but the signal that triggers it is poorly understood in gram-negative bacteria. F …
Structural basis of specific TraD–TraM recognition during F plasmid‐mediated bacterial conjugation
F plasmid‐mediated bacterial conjugation requires interactions between a relaxosome
component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the …
component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the …
A rapid screen for functional mutants of TraM, an autoregulatory protein required for F conjugation
J Lu, RA Fekete, LS Frost - Molecular Genetics and Genomics, 2003 - Springer
TraM is an autoregulatory protein required for conjugative transfer of the F plasmid. A rapid
screening procedure was developed to select for traM mutants constructed by random PCR …
screening procedure was developed to select for traM mutants constructed by random PCR …
Evidence for a monomeric intermediate in the reversible unfolding of F factor TraM
DL Miller, JF Schildbach - Journal of Biological Chemistry, 2003 - ASBMB
F factor TraM is essential for efficient bacterial conjugation, but its molecular function is not
clear. Because the physical properties of TraM may provide clues to its role in conjugation …
clear. Because the physical properties of TraM may provide clues to its role in conjugation …
Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the …
A Beranek, M Zettl, K Lorenzoni, A Schauer… - Journal of …, 2004 - Am Soc Microbiol
Coupling proteins (CPs) are present in type IV secretion systems of plant, animal, and
human pathogens and are essential for DNA transfer in bacterial conjugation systems. CPs …
human pathogens and are essential for DNA transfer in bacterial conjugation systems. CPs …
The essential transfer protein TraM binds to DNA as a tetramer
P Verdino, W Keller, H Strohmaier, K Bischof… - Journal of Biological …, 1999 - ASBMB
The TraM proteins encoded by F-like plasmids are sequence specific DNA binding proteins
that are essential for conjugative DNA transfer. We investigated the quarternary structure …
that are essential for conjugative DNA transfer. We investigated the quarternary structure …