The mechanism of 2, 2, 2-trichloroacetic acid-induced protein precipitation

T Sivaraman, TKS Kumar, G Jayaraman… - Journal of protein chemistry, 1997 - Springer
Abstract The mechanism of 2, 2, 2-trichloroacetic acid (TCA)-induced precipitation of
proteins is studied. The TCA-induced protein precipitation curves are observed to be U …

Trichloroacetic acid‐induced protein precipitation involves the reversible association of a stable partially structured intermediate

D Rajalingam, C Loftis, JJ Xu, TKS Kumar - Protein science, 2009 - Wiley Online Library
Sample preparation for proteomic analysis involves precipitation of protein using 2, 2, 2‐
trichloroacetic acid (TCA). In this study, we examine the mechanism of the TCA‐induced …

Identification of" molten globule"-like state in an all β-sheet protein

TKS Kumar, G Jayaraman, CS Lee, T Sivaraman… - Biochemical and …, 1995 - Elsevier
The cardiotoxin analogue III (CTX III), isolated from the Taiwan Cobra venom (Naja naja
atra), is a sixty amino acid, all β-sheet protein. The 2, 2, 2-trifluoro ethanol (TFE) induced …

2, 2, 2-Trifluoroethanol induces helical conformation in an all β-sheet protein

G Jayaraman, TKS Kumar, AI Arunkumar… - … and biophysical research …, 1996 - Elsevier
The effect of 2, 2, 2-trifluoroethanol (TFE) on the structure of an all β-sheet protein,
cardiotoxin analogue II (CTX II), from the Taiwan cobra (Naja naja atra) is studied. Using …

The role of acidic amino acid residues in the structural stability of snake cardiotoxins

CM Chiang, SL Chang, H Lin, W Wu - Biochemistry, 1996 - ACS Publications
We have recently shown that membrane-related activities of cardiotoxin V from Naja naja
atra (CTX A5) are diminished at acidic pH although the overall β-sheet structure of the …

Characterization of a partially structured state in an all-β-sheet protein

T SIVARAMAN, TKS KUMAR… - Biochemical …, 1997 - portlandpress.com
Cardiotoxin analogue III (CTX III) is a low-molecular-mass all-α-sheet protein isolated from
the Taiwan cobra (Naja naja atra) venom. A stable partially structured state similar to the …

Structure-function relationships in the inorganic salt-induced precipitation of α-chymotrypsin

TM Przybycien, JE Bailey - Biochimica et Biophysica Acta (BBA)-Protein …, 1989 - Elsevier
Abstract α-Chymotrypsin (αCT) was used as a model protein to study the effects of salt-
induced precipitation on protein conformation. Process parameters investigated included the …

Events in the kinetic folding pathway of a small, all β-sheet protein

T Sivaraman, TKS Kumar, DK Chang, WY Lin… - Journal of Biological …, 1998 - ASBMB
The folding of cardiotoxin analogue III (CTX III), a small (60 amino acids), all β-sheet protein
from the venom of the Taiwan Cobra (Naja naja atra) is here investigated. The folding …

Membrane binding motif of the P-type cardiotoxin

PV Dubovskii, DV Dementieva, EV Bocharov… - Journal of Molecular …, 2001 - Elsevier
Carditoxins (CTXs) from cobra snake venoms, the basic 60–62 residue all-beta sheet
polypeptides, are known to bind to and impair the function of cell membranes. To assess the …

Role of glycosphingolipid conformational change in membrane pore forming activity of cobra cardiotoxin

SC Tjong, PL Wu, CM Wang, WN Huang, NL Ho… - Biochemistry, 2007 - ACS Publications
The major cardiotoxin from Taiwan cobra (CTX A3) is a pore forming β-sheet polypeptide
that requires sulfatide (sulfogalactosylceramide, SGC) on the plasma membrane of …